| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bachman, Hunter F (15)
|
MAACWS |
21.73 |
22.17 |
0.44 |
| 1 |
Beattie, Jack F (15)
|
MAWASC |
25.09 |
24.96 |
-0.13 |
| 1 |
West, Ryan C (15)
|
MAHAY |
27.83 |
26.88 |
-0.95 |
| 2 |
DeAngelo II, Nicholas A (18)
|
MAGETY |
22.48 |
22.78 |
0.30 |
| 2 |
Hartranft, Dawson L (15)
|
MAFSSC |
25.96 |
25.40 |
-0.56 |
| 2 |
Risser, Samuel S (15)
|
MANHAC |
28.04 |
27.21 |
-0.83 |
| 3 |
Houck, Jacob A (15)
|
MAEAC |
22.65 |
22.95 |
0.30 |
| 3 |
Block, Auden R (16)
|
MALANY |
25.60 |
25.50 |
-0.10 |
| 3 |
Wingert, Markus J (11)
|
MAHAC |
32.53 |
31.27 |
-1.26 |
| 4 |
Azzalina, Ben W (15)
|
MAEAC |
23.36 |
23.37 |
0.01 |
| 4 |
Toth, Spencer P (15)
|
MAHAY |
26.24 |
25.51 |
-0.73 |
| 4 |
Santos, Marco D (9)
|
MACVAC |
35.95 |
34.84 |
-1.11 |
| 5 |
Andrews, Nikita S (16)
|
MAHAC |
23.34 |
23.72 |
0.38 |
| 5 |
Chappell, Benjamin J (15)
|
MAHAC |
26.05 |
26.33 |
0.28 |
| 6 |
Yengo, Jack C (18)
|
MAPACS |
24.40 |
24.99 |
0.59 |
| 6 |
Besnoff, Tyler I (16)
|
MALAC |
25.43 |
26.55 |
1.12 |
| 7 |
Fisher, Garrett M (17)
|
MANHAC |
24.08 |
25.22 |
1.14 |
| 7 |
Richardson, Titus (16)
|
MACYA |
26.75 |
26.59 |
-0.16 |
| 8 |
Clough, Colin T (13)
|
MAHAC |
24.74 |
25.25 |
0.51 |
| 8 |
Brandsema, Luke A (15)
|
MAFSSC |
25.78 |
26.64 |
0.86 |
|
Horton, Ajay (15)
|
MAACWS |
26.02 |
DQ |
- |