| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Boyer, Chloe G (9)
|
NIISWM |
46.72 |
45.53 |
-1.19 |
| 2 |
Ramos, Daniela I (9)
|
NIUNAC |
46.64 |
46.94 |
0.30 |
| 3 |
Ivery, Allison L (9)
|
NICSCI |
49.96 |
48.84 |
-1.12 |
| 4 |
Adams, Kayleigh C (9)
|
NICSCI |
49.39 |
49.64 |
0.25 |
| 5 |
Dardis, Gabriella A (9)
|
NISYCH |
51.80 |
50.05 |
-1.75 |
| 6 |
Murphy, Gabriela (9)
|
NIKRKN |
49.27 |
50.37 |
1.10 |
| 7 |
El Ghissassi, Sidra S (8)
|
NIVEST |
51.69 |
50.46 |
-1.23 |
| 8 |
Denlinger, Elizabeth J (9)
|
NIWAAC |
52.78 |
51.79 |
-0.99 |
| 9 |
Wang, Bethany B (6)
|
NIKRKN |
50.75 |
52.80 |
2.05 |
| 10 |
Oliveira, Mariana P (8)
|
NISYCH |
51.81 |
55.07 |
3.26 |