| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cheresnowsky, Olivia K (9)
|
NIWAAC |
36.32 |
35.24 |
-1.08 |
| 2 |
El Ghissassi, Sidra S (8)
|
NIVEST |
36.15 |
35.73 |
-0.42 |
| 3 |
Dardis, Gabriella A (9)
|
NISYCH |
36.05 |
35.92 |
-0.13 |
| 4 |
Ramos, Daniela I (9)
|
NIUNAC |
36.82 |
36.38 |
-0.44 |
| 5 |
Akley, Isabelle A (9)
|
NIJETS |
38.37 |
36.44 |
-1.93 |
| 6 |
Owczarzak, Madeline L (9)
|
NICSCI |
42.35 |
36.66 |
-5.69 |
| 7 |
Goeckel, Catherine G (9)
|
NIBSSC |
38.71 |
38.09 |
-0.62 |
| 8 |
Ivery, Allison L (9)
|
NICSCI |
38.14 |
38.47 |
0.33 |
| 9 |
Fuller, Anna G (9)
|
NISYCH |
36.93 |
38.53 |
1.60 |
| 10 |
Adams, Kayleigh C (9)
|
NICSCI |
38.08 |
39.10 |
1.02 |
| 11 |
Leonard, Kaitllyn S (9)
|
NIWAAC |
42.52 |
41.45 |
-1.07 |
| 12 |
Kondrup, Kenzington A (9)
|
NIUNAC |
42.34 |
42.24 |
-0.10 |
| 13 |
Zuckerman, Zoe R (9)
|
NICSCI |
42.77 |
42.75 |
-0.02 |
| 14 |
Devendorf, Madelyn G (9)
|
NIJETS |
41.21 |
43.20 |
1.99 |