| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Helminiak, Lauren M (12)
|
MDMAS |
24.93 |
24.88 |
-0.05 |
| 2 |
Helminiak, Katherine E (15)
|
MDMAS |
25.23 |
25.11 |
-0.12 |
| 3 |
Nwaeze, Zuilda N (13)
|
MDCAA! |
25.16 |
25.35 |
0.19 |
| 4 |
Hay, Louise J (16)
|
MARAD |
25.25 |
25.47 |
0.22 |
| 5 |
Oechsli, Saige M (17)
|
MDHFY |
25.71 |
25.91 |
0.20 |
| 6 |
Rothrock, Jessica L (14)
|
MDCAA! |
25.70 |
25.93 |
0.23 |
| 7 |
Catrambone, Carolyn E (16)
|
MDACA |
25.96 |
26.08 |
0.12 |
| 8 |
Cowperthwaite, Riley E (15)
|
MDFSC |
25.47 |
26.09 |
0.62 |
| 9 |
Czaja, Sophia H (18)
|
MDFSC |
25.60 |
26.12 |
0.52 |
| 10 |
Townsend, Ashliegh C (14)
|
MDHFY |
25.70 |
26.61 |
0.91 |
| 11 |
Green-Williams, Pauline (15)
|
PVDCPR |
26.57 |
26.73 |
0.16 |
| 12 |
Heyman, Selby E (15)
|
VADIG |
25.34 |
26.83 |
1.49 |
| 13 |
Corley, Sydney G (13)
|
MDCAA! |
27.12 |
26.98 |
-0.14 |
| 14 |
Luljak, Julia C (17)
|
MDMSC |
26.71 |
27.45 |
0.74 |
| 15 |
Demkowski, Kelli A (16)
|
VADIG |
27.81 |
27.77 |
-0.04 |
|
Handal, Isabel L (15)
|
MARAD |
24.85 |
DQ |
- |