| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Graham, Jami L (8)
|
MDFSC |
41.58 |
39.01 |
-2.57 |
| 2 |
Dixon, Madelyn M (7)
|
MDHOCO |
45.20 |
40.80 |
-4.40 |
| 3 |
Deoliveira, Mariana K (8)
|
MDHOCO |
44.10 |
41.09 |
-3.01 |
| 4 |
Trionfo, Giavanna E (8)
|
MDACA |
41.64 |
43.97 |
2.33 |
| 5 |
Golembiesky, Adeline F (8)
|
MDLBA |
46.64 |
44.49 |
-2.15 |
| 7 |
Houghton, Brynn G (8)
|
MDSPRC |
48.69 |
45.18 |
-3.51 |
| 8 |
Setzfand, Eliza Jane H (8)
|
MDCAC |
45.31 |
46.49 |
1.18 |
| 9 |
Jones, Chloe T (8)
|
MDFMSC |
53.17 |
47.24 |
-5.93 |
| 10 |
Sharif-Jackson, Zahara I (7)
|
MDBCSC |
|
49.32 |
- |
| 11 |
Pinheiro, Ryan E (8)
|
MDLBA |
58.50 |
52.41 |
-6.09 |
| 12 |
Blaha, Sienna K (8)
|
MDEST |
55.98 |
53.14 |
-2.84 |
| 13 |
Yang-He, Kate Y (7)
|
MDHOCO |
|
54.11 |
- |
| 14 |
Perich, Cecelia J (8)
|
MDLBA |
|
57.81 |
- |
| 15 |
Duren, Jayla T (8)
|
MDBCSC |
54.81 |
57.91 |
3.10 |
| 16 |
Devine, Madeline G (6)
|
MDFSC |
|
59.21 |
- |
| 17 |
Wiseman, Ellyse C (7)
|
MDLBA |
57.29 |
59.24 |
1.95 |
| 18 |
Bolton, Ama L (8)
|
MDFSC |
|
1:02.40 |
- |
| 19 |
Mets, Anna (6)
|
MDMAS |
|
1:04.27 |
- |
|
He, Viola (8)
|
MDMAS |
|
DQ |
- |
|
Betley, Addison E (8)
|
MDASC |
|
DQ |
- |
|
Belt, Sydney E (8)
|
MDMAS |
|
NS |
- |