| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ismailova, Diana (10)
|
VAGATR |
3:04.92 |
2:43.87 |
-21.05 |
| 2 |
Fernando, Scarlett O (10)
|
NCEAC |
3:05.90 |
2:48.93 |
-16.97 |
| 3 |
Lickfold, Katelyn D (10)
|
NCRSA |
2:48.78 |
2:49.32 |
0.54 |
| 4 |
Murtaugh, Lindsay E (10)
|
VAGATR |
3:02.61 |
2:52.64 |
-9.97 |
| 5 |
Carter, Emmi D (10)
|
VACCA |
3:26.48 |
2:56.20 |
-30.28 |
| 6 |
Sylvester-Johnson, Jillian B (10)
|
VAGATR |
3:12.58 |
2:56.57 |
-16.01 |
| 7 |
Hadsell, Meghan L (10)
|
NCRSA |
3:02.01 |
3:00.78 |
-1.23 |
| 8 |
Starr, Allison P (10)
|
VAHOKI |
|
3:09.50 |
- |
| 9 |
Dellinger, Evelyn K (10)
|
SEBSC |
3:53.71 |
3:12.55 |
-41.16 |
| 10 |
Miller, Maris R (10)
|
NCRSA |
|
3:15.84 |
- |
| 11 |
Johnson, Madison G (10)
|
SEBSC |
3:33.11 |
3:25.81 |
-7.30 |
| 12 |
Hennaux, Eva D (10)
|
SEKP |
3:41.23 |
3:36.99 |
-4.24 |