| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lugar, George C (8)
|
VAGATR |
50.71 |
47.38 |
-3.33 |
| 2 |
Cummins, Bodhi K (7)
|
NCEAC |
55.73 |
54.10 |
-1.63 |
| 3 |
Brumback, Noah G (8)
|
NCRSA |
55.46 |
54.65 |
-0.81 |
| 4 |
Strupe, Fredric J (8)
|
NCTEAM |
57.97 |
54.72 |
-3.25 |
| 5 |
Nunn, Bo B (7)
|
NCEAC |
54.41 |
56.50 |
2.09 |
| 6 |
Starr, Bryson S (8)
|
VAHOKI |
|
58.20 |
- |
| 7 |
Du, Andrew Z (8)
|
VAHOKI |
|
1:02.01 |
- |
| 8 |
Harris, Anderson H (7)
|
NCTEAM |
|
1:02.67 |
- |
| 9 |
Feathers, Jacob M (8)
|
SEKP |
|
1:02.89 |
- |
| 10 |
Hoyos, Benjamin T (8)
|
VASCAT |
1:22.86 |
1:05.18 |
-17.68 |
| 11 |
Pereira, Jackson E (7)
|
NCRSA |
|
1:14.82 |
- |
| 12 |
Durbin, Finnegan P (8)
|
VAHOKI |
|
1:17.46 |
- |
|
Strupe, Bayer M (6)
|
NCTEAM |
|
NS |
- |
|
Giovannini, Niccolo J (8)
|
VAUN-G |
|
NS |
- |