| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Callahan, Grace H (11)
|
PVMACH |
33.15 |
32.13 |
-1.02 |
| 2 |
Wilhite, Charlotte L (11)
|
PVMACH |
33.13 |
32.17 |
-0.96 |
| 3 |
Nichols, Cassandra L (11)
|
NCRSA |
33.62 |
32.46 |
-1.16 |
| 4 |
Jones, Madison O (11)
|
NCEAC |
33.83 |
33.11 |
-0.72 |
| 5 |
Grim, Evelyn M (11)
|
VAHOKI |
34.78 |
33.23 |
-1.55 |
| 6 |
Spivey, Tia F (11)
|
SEBSC |
33.97 |
33.52 |
-0.45 |
| 7 |
Baldwin, Ava S (11)
|
VAHOKI |
37.75 |
34.07 |
-3.68 |
| 8 |
Jones, Layne C (11)
|
VACCA |
|
35.29 |
- |
| 9 |
Bernedo, Helaina S (11)
|
SEBSC |
40.27 |
35.59 |
-4.68 |
| 10 |
Jones, Shelby L (11)
|
VAHOKI |
|
36.38 |
- |
| 11 |
Carter, Maddi G (11)
|
VACCA |
40.27 |
36.59 |
-3.68 |
| 12 |
Sturgill, Lydia K (11)
|
PVMACH |
44.32 |
36.87 |
-7.45 |
| 13 |
Edwards, Natalie M (11)
|
VAHOKI |
|
37.42 |
- |
| 14 |
Viar, Annaliese R (11)
|
VACCA |
39.89 |
38.50 |
-1.39 |
| 15 |
Hedrick, Alexandra T (11)
|
NCTEAM |
|
38.58 |
- |
| 16 |
Sachar, Emma G (11)
|
NCTEAM |
|
40.63 |
- |
| 17 |
Miller, Deanna R (11)
|
SEBSC |
50.58 |
43.29 |
-7.29 |
| 18 |
Fentress, Harmony L (11)
|
VAHOKI |
|
46.75 |
- |
| 19 |
Proco, Chloe A (11)
|
NCEAC |
47.76 |
50.21 |
2.45 |