| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Robinson, Tatum A (14)
|
VAHOKI |
1:24.36 |
1:25.12 |
0.76 |
| 2 |
Falwell, Caroline Y (13)
|
VALY |
1:25.01 |
1:25.76 |
0.75 |
| 3 |
Fuller, Abigail B (14)
|
VAACAC |
1:26.40 |
1:25.92 |
-0.48 |
| 4 |
De Jong, Emma E (14)
|
VAVSTP |
1:26.18 |
1:30.48 |
4.30 |
| 5 |
Foster, Madeline E (13)
|
WVWILD |
1:29.92 |
1:30.74 |
0.82 |
| 6 |
Marotta, Caroline G (14)
|
VALY |
1:32.71 |
1:30.93 |
-1.78 |
| 7 |
Jones, Chelsey M (13)
|
VAVSTP |
1:34.75 |
1:35.02 |
0.27 |
| 8 |
Lawrence, Peyton E (13)
|
VASFY |
1:35.74 |
1:37.06 |
1.32 |
| 9 |
Layne, MacKenzie G (14)
|
WVWILD |
1:36.04 |
1:37.55 |
1.51 |
| 10 |
Jackson, Elizabeth H (13)
|
VAACAC |
|
1:38.75 |
- |
| 11 |
Hatfield, Lindsay L (14)
|
VALY |
1:38.53 |
1:43.31 |
4.78 |
| 12 |
Wagoner, Arleigh K (13)
|
VALY |
|
1:43.62 |
- |
| 13 |
Jenkins, Jaedyn L (13)
|
VASFY |
1:46.00 |
1:44.52 |
-1.48 |
| 14 |
Kawatsky, Abbigail M (13)
|
VAVSTP |
1:36.99 |
1:45.85 |
8.86 |
| 15 |
Bivens, Madison S (13)
|
VALY |
|
1:46.47 |
- |
| 16 |
Schellhammer, Alexis T (13)
|
VAVSTP |
1:42.02 |
1:49.45 |
7.43 |
| 17 |
Realmuto, Keira E (13)
|
VASFY |
|
1:49.92 |
- |
| 18 |
Bauserman, Savannah G (14)
|
VALY |
1:53.28 |
1:50.00 |
-3.28 |