| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scott, Erin G (19)
|
VAUN |
|
34.04 |
- |
| 2 |
Dejager, Caroline D (21)
|
VAUN |
|
35.71 |
- |
| 3 |
Enneking, Tara M (17)
|
VALY |
34.18 |
36.13 |
1.95 |
| 4 |
Plogger, Alexis N (17)
|
VALY |
37.51 |
37.98 |
0.47 |
| 5 |
Haise, Sophie A (15)
|
VAUN-A |
39.69 |
38.49 |
-1.20 |
| 6 |
Childress, Kelsey E (19)
|
VALY |
38.77 |
38.84 |
0.07 |
| 7 |
Gross, Bojena D (17)
|
WVWILD |
40.27 |
39.91 |
-0.36 |
| 8 |
Nussbaum, Lexi J (15)
|
VAHOKI |
47.32 |
40.58 |
-6.74 |
| 9 |
Humphreys, Samantha G (16)
|
WVWILD |
42.32 |
40.66 |
-1.66 |
| 10 |
Erquiaga, Carrington M (17)
|
VAUN-L |
37.99 |
40.72 |
2.73 |
| 11 |
Eckert, Ellisa M (15)
|
VALY |
|
40.73 |
- |
| 12 |
Raines, Regan E (18)
|
WVWILD |
41.88 |
42.03 |
0.15 |
| 13 |
Rowe, Erin (16)
|
WVWILD |
41.51 |
42.48 |
0.97 |
| 14 |
Rogers, Natalie C (15)
|
VALY |
|
43.88 |
- |
| 15 |
van Ek, Kylie (16)
|
VAACAC |
|
45.74 |
- |
| 16 |
Jones, Jordyn C (16)
|
VAVSTP |
50.42 |
46.01 |
-4.41 |
| 17 |
Searwar, Hannah G (17)
|
VALY |
|
54.78 |
- |
|
Pape, Nicole A (19)
|
VAUN |
|
NS |
- |