| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Robinson, Tatum A (14)
|
VAHOKI |
33.28 |
32.65 |
-0.63 |
| 2 |
Kawatsky, Abbigail M (13)
|
VAVSTP |
32.74 |
32.79 |
0.05 |
| 3 |
Thomas, Catherine E (13)
|
VALY |
|
34.09 |
- |
| 4 |
Falwell, Caroline Y (13)
|
VALY |
34.39 |
34.39 |
0.00 |
| 5 |
Marotta, Caroline G (14)
|
VALY |
|
34.55 |
- |
| 6 |
De Jong, Emma E (14)
|
VAVSTP |
51.29 |
35.23 |
-16.06 |
| 7 |
Jackson, Elizabeth H (13)
|
VAACAC |
36.63 |
35.86 |
-0.77 |
| 8 |
Davis, Emma R (13)
|
VAHOKI |
36.98 |
36.05 |
-0.93 |
| 9 |
Warren, Grace K (14)
|
VALY |
|
36.90 |
- |
| 10 |
Russell, Caroline H (14)
|
VALY |
|
37.76 |
- |
| 11 |
Lawrence, Peyton E (13)
|
VASFY |
40.42 |
37.90 |
-2.52 |
| 12 |
Dodge, Sophie G (14)
|
VALY |
|
38.97 |
- |
| 13 |
Wagoner, Arleigh K (13)
|
VALY |
|
42.27 |
- |
| 14 |
Schellhammer, Alexis T (13)
|
VAVSTP |
38.06 |
42.64 |
4.58 |
| 15 |
Jenkins, Jaedyn L (13)
|
VASFY |
48.32 |
43.86 |
-4.46 |
| 16 |
Hatfield, Lindsay L (14)
|
VALY |
45.26 |
45.33 |
0.07 |
| 17 |
Bivens, Madison S (13)
|
VALY |
|
47.55 |
- |