| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
English, Penelope L (9)
|
MAFCA |
3:29.28 |
2:53.76 |
-35.52 |
| 1 |
Wolbert, Sydney R (9)
|
MAPFY |
2:57.78 |
2:55.40 |
-2.38 |
| 2 |
Kushner, Emma M (10)
|
MAPSC |
3:08.73 |
2:57.04 |
-11.69 |
| 2 |
Murphy, Ciara J (10)
|
MAKIDS |
3:30.70 |
3:15.21 |
-15.49 |
| 3 |
Coombe, Taylor B (10)
|
MAPSC |
3:04.90 |
3:00.97 |
-3.93 |
| 3 |
Harmon, Sophia D (10)
|
MAEMAC |
|
3:16.32 |
- |
| 4 |
Pappentick, Addison K (10)
|
MAEMAC |
3:13.96 |
3:14.36 |
0.40 |
| 4 |
Lamm, Ava R (10)
|
MAEMAC |
3:39.50 |
3:50.59 |
11.09 |
|
Kehoe-Huck, Adelaide N (9)
|
MAPSC |
|
DQ |
- |
|
McPeek, Kelsey M (9)
|
MAPSC |
|
DQ |
- |