| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bagley, Jaden (7)
|
MASAKA |
42.57 |
41.23 |
-1.34 |
| 2 |
Roser, Matthew W (7)
|
MAGPAC |
43.26 |
43.31 |
0.05 |
| 3 |
Walstrom, Seamus D (7)
|
MADST |
46.74 |
44.78 |
-1.96 |
| 4 |
Bickett, Luke T (7)
|
MAGPAC |
42.74 |
45.98 |
3.24 |
| 5 |
Ajunwa, Donovan C (7)
|
MAUDAC |
43.65 |
46.04 |
2.39 |
| 6 |
Maier, Carter J (7)
|
MAGPAC |
46.37 |
47.03 |
0.66 |
| 7 |
Luo, James H (7)
|
MASSC |
52.37 |
47.99 |
-4.38 |
| 8 |
Shapiro, Samuel B (7)
|
MAUDAC |
48.25 |
48.25 |
0.00 |
| 9 |
Pawlikowski, Jacob N (7)
|
MAECCC |
52.69 |
48.33 |
-4.36 |
| 10 |
Burkhardt, James (7)
|
MANSC |
56.00 |
48.65 |
-7.35 |
| 11 |
Gursky, Solomon B (7)
|
MAFCA |
50.30 |
49.20 |
-1.10 |
| 12 |
Nixon, Noble J (7)
|
MAFCA |
51.29 |
49.84 |
-1.45 |
| 13 |
Waggoner, Gabriel J (7)
|
MAJW |
48.21 |
50.51 |
2.30 |
| 14 |
Petrakis, Michael G (7)
|
MAUN4 |
1:06.22 |
50.85 |
-15.37 |
| 15 |
Pierce, Parker M (7)
|
MAUDAC |
51.49 |
51.74 |
0.25 |
| 16 |
Smyth, John W (7)
|
MADST |
1:00.63 |
52.82 |
-7.81 |
| 17 |
Stankiewicz-Goldsmit, Willia (7)
|
MAFCA |
47.79 |
52.84 |
5.05 |
| 18 |
Xun, Changyuan L (7)
|
MADST |
53.81 |
53.09 |
-0.72 |
| 19 |
Shaffer, Owen C (7)
|
MAGPAC |
58.44 |
53.14 |
-5.30 |
| 20 |
Papadopoulos, Fotios S (7)
|
MATRI |
1:04.40 |
54.98 |
-9.42 |
| 21 |
McDermott, Declan L (7)
|
MAGRA |
1:04.09 |
55.20 |
-8.89 |
| 22 |
Andryca, Derek P (7)
|
MAWAVE |
55.81 |
56.50 |
0.69 |
| 23 |
Paoletti, Philip N (7)
|
MADST |
53.19 |
56.99 |
3.80 |
| 24 |
Everingham, Bryce A (7)
|
MAGPAC |
54.36 |
57.03 |
2.67 |
| 25 |
Pagano, Vincent S (7)
|
MALAC |
57.91 |
57.96 |
0.05 |
| 26 |
Singer, Daniel D (7)
|
MATWAC |
1:06.67 |
1:00.84 |
-5.83 |
| 27 |
Dong, Keith H (7)
|
MAFCA |
1:39.02 |
1:01.64 |
-37.38 |
| 28 |
Carter, James (7)
|
MAWA |
1:01.57 |
1:05.19 |
3.62 |
| 29 |
Garrido, Evan T (7)
|
MAGAAC |
1:17.66 |
1:06.52 |
-11.14 |
|
Smith, Joshua D (7)
|
MAGPAC |
1:08.96 |
DQ |
- |