| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blough, Madelyn G (10)
|
MAKA |
31.56 |
30.60 |
-0.96 |
| 1 |
Livingston, Claire (10)
|
MAJY |
34.43 |
32.57 |
-1.86 |
| 1 |
Ivall, Olivia P (10)
|
MDFAST |
38.07 |
36.34 |
-1.73 |
| 2 |
Schaeberle, Claire E (10)
|
MAUN-0 |
34.27 |
32.63 |
-1.64 |
| 2 |
Perry, Samantha J (10)
|
MDFAST |
37.67 |
37.04 |
-0.63 |
| 3 |
Harshman, Madeline M (10)
|
MDFAST |
34.90 |
32.97 |
-1.93 |
| 3 |
Brashear, Johanna H (10)
|
MDFAST |
36.73 |
37.11 |
0.38 |
| 4 |
Hancock, Caroline R (10)
|
MACVAC |
33.28 |
33.62 |
0.34 |
| 4 |
Coombs, Emily L (10)
|
MAKA |
42.31 |
37.21 |
-5.10 |
| 5 |
Gilsinn, Laila N (10)
|
MDFAST |
42.96 |
39.10 |
-3.86 |
| 6 |
Nguyen, Sofie L (9)
|
MACVAC |
|
39.83 |
- |
| 7 |
Dabrowski, Sarah R (9)
|
MAKA |
|
44.55 |
- |
| 8 |
Orcutt, Violet G (9)
|
MDFAST |
50.77 |
46.11 |
-4.66 |