| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mellinger, Ashley E (10)
|
MALAC |
2:34.23 |
2:30.30 |
-3.93 |
| 2 |
Scime, Alex A (10)
|
MAGRA |
2:40.32 |
2:36.37 |
-3.95 |
| 3 |
Petrakis, Grace L (10)
|
MACBFY |
2:41.71 |
2:37.41 |
-4.30 |
| 4 |
Monti, Lila R (10)
|
MRTVSC |
2:44.62 |
2:39.22 |
-5.40 |
| 5 |
Retzlaff, Claudia L (10)
|
MRTVSC |
2:43.27 |
2:40.14 |
-3.13 |
| 6 |
Vinciguerra, Jacqueline X (10)
|
MRTVSC |
2:51.87 |
2:41.35 |
-10.52 |
| 7 |
Nassar, Noor (10)
|
MRLGAC |
2:51.31 |
2:42.26 |
-9.05 |
| 8 |
Blough, Madelyn G (10)
|
MAKA |
2:44.04 |
2:42.87 |
-1.17 |
| 9 |
Parry, Mia J (10)
|
MALAC |
2:46.27 |
2:44.50 |
-1.77 |
| 10 |
Vavalle, Giuliana R (10)
|
MRTVSC |
3:09.98 |
2:45.14 |
-24.84 |
| 11 |
Wigginton, Samantha L (10)
|
MAHHAA |
2:51.51 |
2:46.31 |
-5.20 |
| 12 |
Mahler, Olivia A (10)
|
MAHAC |
2:50.28 |
2:46.62 |
-3.66 |
| 13 |
Hickey, Lillian G (10)
|
MALMAC |
2:45.14 |
2:47.46 |
2.32 |
| 14 |
Washington, Amira (10)
|
MRTRI |
2:52.05 |
2:48.02 |
-4.03 |
| 15 |
Ma, Vivian (10)
|
CTRAC |
2:56.02 |
2:50.52 |
-5.50 |
| 16 |
Rodgers, Mirabel G (10)
|
CTRAC |
2:54.66 |
2:53.92 |
-0.74 |
| 17 |
Grawl, Trina M (10)
|
MASEA |
2:53.32 |
2:54.21 |
0.89 |