| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Barry, Patrick J (8)
|
MAMSA |
47.80 |
45.30 |
-2.50 |
| 2 |
Markouski, Timophey V (8)
|
MAUN-M |
47.64 |
45.46 |
-2.18 |
| 3 |
Tiankwa, Cyrian E (8)
|
MASSC |
|
46.10 |
- |
| 4 |
Kim, Caleb R (8)
|
MAJW |
|
46.30 |
- |
| 5 |
Sharp, Brenden J (8)
|
MAJW |
|
49.68 |
- |
| 6 |
Giordano, Ryan B (8)
|
MAJW |
54.56 |
52.54 |
-2.02 |
| 7 |
Yerkes, Jacob M (8)
|
MAJW |
1:14.84 |
53.66 |
-21.18 |
| 8 |
Duncan, Connor A (8)
|
MAJW |
|
54.57 |
- |
| 9 |
Broderick, Ronan S (8)
|
MAJW |
|
56.66 |
- |
| 10 |
Waggoner, Gabriel J (7)
|
MAJW |
|
56.94 |
- |
| 11 |
Luo, James H (7)
|
MASSC |
59.64 |
57.36 |
-2.28 |
| 12 |
Levy, Dylan P (8)
|
MAJW |
|
1:01.02 |
- |
| 13 |
Vinod, Siddharth (7)
|
MAJW |
|
1:03.94 |
- |
|
Yu, Evan H (8)
|
MAMSA |
|
DQ |
- |
|
Schneider, Sebastian R (8)
|
MAJW |
|
NS |
- |