| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Klaehn, Milla (7)
|
MAUN-E |
36.21 |
18.73 |
-17.48 |
| 2 |
Bobalik, Vivian R (7)
|
MAGRA |
20.67 |
19.90 |
-0.77 |
| 3 |
Grossi, Giuliana R (7)
|
MAJW |
27.14 |
24.06 |
-3.08 |
| 4 |
Eruslanova, Elizabeth (7)
|
MASSC |
24.18 |
24.88 |
0.70 |
| 5 |
Laaguiby, Sophia (7)
|
MABSC |
27.18 |
25.40 |
-1.78 |
| 6 |
Murray, Ellie R (7)
|
MAGAAC |
35.82 |
26.68 |
-9.14 |
| 7 |
Vitarelli, Eden J (7)
|
MAJW |
30.01 |
28.26 |
-1.75 |
| 8 |
Halligan, Molly E (7)
|
MAGAAC |
|
28.74 |
- |
| 9 |
Burris, Gwendolyn F (7)
|
MAFCA |
|
28.77 |
- |
| 10 |
Vogl, Ilana C (7)
|
MAFCA |
|
29.28 |
- |
| 11 |
Stibler, Olivia Q (7)
|
MAJW |
40.41 |
29.55 |
-10.86 |
| 12 |
Shaud, Audrey A (7)
|
MASSC |
31.11 |
30.12 |
-0.99 |
| 13 |
Cantor, Avery E (7)
|
MABSC |
28.72 |
30.29 |
1.57 |
| 14 |
Martin, Charlotte E (7)
|
MAFCA |
|
31.16 |
- |
| 15 |
Lightman, Chloe A (7)
|
MABSC |
|
32.56 |
- |
| 16 |
Zarzecki, Madison V (7)
|
MAJW |
33.71 |
36.36 |
2.65 |
| 17 |
MacKenzie, Beatrice G (7)
|
MABSC |
31.57 |
38.78 |
7.21 |
| 18 |
Baror, Maya (7)
|
MAFCA |
|
43.59 |
- |
|
Rosenberg, Reese F (7)
|
MAFCA |
30.93 |
DQ |
- |
|
Nalawde, Aasheeta A (7)
|
MAJW |
|
DQ |
- |
|
Ojha, Elina A (7)
|
MAJW |
51.46 |
DQ |
- |