| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kalinowski, Thomas J (5)
|
MAWAC |
26.22 |
26.19 |
-0.03 |
| 2 |
Poole, Grayson R (6)
|
MAWAC |
24.98 |
26.25 |
1.27 |
| 3 |
Enakhimion, Makinde N (5)
|
MAEAAC |
27.04 |
26.94 |
-0.10 |
| 4 |
Sullivan, Sawyer B (6)
|
MAGRA |
29.32 |
27.18 |
-2.14 |
| 5 |
Roop VI, Albert J (6)
|
MAWAC |
31.09 |
29.85 |
-1.24 |
| 6 |
Fox, William K (6)
|
MAWAC |
34.26 |
30.63 |
-3.63 |
| 7 |
Armstrong, Christopher J (6)
|
MAWA |
31.00 |
31.16 |
0.16 |
| 8 |
Scime, William E (5)
|
MAGRA |
33.56 |
32.19 |
-1.37 |
| 9 |
Rigney, William T (5)
|
MAWAC |
34.31 |
32.52 |
-1.79 |
| 10 |
Gilbert, Sean M (6)
|
MAWA |
|
32.55 |
- |
| 11 |
Waldbuesser, August F (6)
|
MAEAAC |
33.24 |
32.86 |
-0.38 |
| 12 |
Haynes, Michael J (5)
|
MAWAC |
|
32.88 |
- |
| 13 |
Ying, Matthew Y (6)
|
MAMSA |
33.09 |
32.98 |
-0.11 |
| 14 |
Li, Mason J (6)
|
MASSC |
|
35.40 |
- |
| 15 |
Fender, Luke C (5)
|
MAMSA |
|
37.14 |
- |
| 16 |
Taggart, Nathan R (5)
|
MAWAC |
|
42.89 |
- |
| 17 |
Kanabar, Aaryan M (6)
|
MAACAC |
|
48.13 |
- |
| 18 |
Connell, Brody R (4)
|
MAWAC |
46.53 |
56.97 |
10.44 |
|
Ewer, Maxim P (5)
|
MAMSA |
36.15 |
DQ |
- |
|
Sambuco, Mason J (6)
|
MAGRA |
|
DQ |
- |
|
Whomsley, Liam M (6)
|
MAWA |
35.80 |
NS |
- |
|
Deaver, Matthew C (6)
|
MAGRA |
37.45 |
NS |
- |