| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hickey, Lillian G (10)
|
MATRI |
30.30 |
34.84 |
4.54 |
| 2 |
Mottershead, Freya L (10)
|
MACBFY |
30.33 |
35.32 |
4.99 |
| 3 |
Smallwood, Charlotte R (9)
|
MATRI |
32.88 |
37.44 |
4.56 |
| 4 |
Coombe, Taylor B (10)
|
MAPSC |
33.87 |
38.00 |
4.13 |
| 5 |
Z'Ningray, Tenzee N (9)
|
MATRI |
38.79 |
42.12 |
3.33 |
| 6 |
Krause, Mabry C (9)
|
MAPSC |
39.19 |
43.58 |
4.39 |
| 7 |
Kim, Sarang R (9)
|
MANPAC |
41.45 |
44.45 |
3.00 |
| 8 |
Baughman, Caroline E (9)
|
MAUDAC |
38.51 |
46.20 |
7.69 |
| 9 |
Clark, Gabriella A (9)
|
MALMOR |
40.41 |
46.77 |
6.36 |
| 9 |
Dai, Ariana (9)
|
MAUDAC |
33.70 |
46.77 |
13.07 |
|
Hahn, Milla G (10)
|
MAPSC |
29.83 |
NS |
- |
|
Zhang, Grace L (10)
|
MAPSC |
34.74 |
NS |
- |