| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ford, Garrity A (12)
|
MAPAAC |
45.16 |
44.46 |
-0.70 |
| 2 |
Magditch, Georgia E (12)
|
MAPAAC |
51.45 |
48.16 |
-3.29 |
| 3 |
Cheplick, Natalie A (11)
|
MAPAAC |
46.65 |
48.29 |
1.64 |
| 4 |
Robinson, Grace C (12)
|
MAEMAC |
|
52.60 |
- |
| 5 |
Pappentick, Addison K (11)
|
MAEMAC |
52.89 |
54.51 |
1.62 |
| 6 |
Fry, Genevieve A (12)
|
MAEMAC |
52.24 |
54.68 |
2.44 |
| 7 |
Shutkufski, Madison H (11)
|
MAEMAC |
|
54.99 |
- |
| 8 |
Daly, Harper O (12)
|
MAEMAC |
|
56.01 |
- |
| 9 |
Laski, Emilia C (9)
|
MAEMAC |
50.94 |
56.18 |
5.24 |
| 10 |
Adams, Sara G (11)
|
MAEMAC |
|
56.75 |
- |
| 11 |
Evans, Tegan R (9)
|
MAPAAC |
1:04.59 |
1:03.40 |
-1.19 |