| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
King, Avery O (14)
|
MAEMAC |
5:25.78 |
5:17.78 |
-8.00 |
| 2 |
Iordanov, Monica I (13)
|
MASEA |
5:42.68 |
5:37.84 |
-4.84 |
| 3 |
Miller, Ayala (13)
|
MAUMLY |
5:40.79 |
5:43.91 |
3.12 |
| 4 |
Haas, Keira M (13)
|
MAUMLY |
5:56.69 |
5:46.36 |
-10.33 |
| 5 |
Rossi, Isabella M (13)
|
MASEA |
5:55.06 |
5:51.36 |
-3.70 |
| 6 |
Wiley, Keira E (14)
|
MAUMLY |
6:08.91 |
5:54.85 |
-14.06 |
| 7 |
Tran, Melissa (13)
|
MAPVA |
5:45.00 |
5:55.86 |
10.86 |
| 8 |
Gaskill, Grace I (13)
|
MAPVA |
6:00.43 |
6:02.87 |
2.44 |
| 9 |
Iacobelli, Sophia C (14)
|
MAEMAC |
6:05.44 |
6:03.59 |
-1.85 |
| 10 |
Johnson, Natalie M (13)
|
MAWAC |
5:55.50 |
6:09.69 |
14.19 |
| 11 |
Danastasio, Giovanna M (13)
|
MAWAVE |
5:57.79 |
6:14.83 |
17.04 |
| 12 |
Schober, Lauren E (14)
|
MAUNPV |
6:30.43 |
6:19.55 |
-10.88 |
| 13 |
Shawaryn, Stephanie A (13)
|
MAYBCC |
6:04.00 |
6:25.51 |
21.51 |
| 14 |
Johnson, Isabelle C (13)
|
MAEMAC |
6:44.89 |
6:27.22 |
-17.67 |
| 15 |
Hark, Abigail J (14)
|
MAEMAC |
6:51.14 |
6:28.83 |
-22.31 |
| 16 |
Schaffer, Caroline R (14)
|
MAEMAC |
6:53.19 |
6:30.99 |
-22.20 |
| 17 |
Sakers, Alexis R (14)
|
MADST |
6:23.48 |
6:37.54 |
14.06 |
| 18 |
Gould, Morgan G (14)
|
MAEMAC |
7:04.56 |
6:50.78 |
-13.78 |
| 19 |
Braswell, Olivia (13)
|
MAUMLY |
6:59.07 |
6:52.70 |
-6.37 |
| 20 |
Brewer, Veronica L (14)
|
MAUMLY |
7:06.51 |
7:07.39 |
0.88 |