| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shanor, Kaytin J (7)
|
GUSSAN |
43.09 |
44.25 |
1.16 |
| 2 |
Yang, Chloe Y (7)
|
GUSSAN |
44.43 |
46.73 |
2.30 |
| 3 |
Griffin-Coyle, Addyson G (7)
|
GUMAC |
51.42 |
47.88 |
-3.54 |
| 4 |
Herbel, Carissa M (7)
|
GUSSAN |
47.90 |
48.55 |
0.65 |
| 5 |
Dulworth, Ella M (7)
|
GUSSAN |
47.79 |
51.37 |
3.58 |
| 6 |
VanVossen, Kathryn G (7)
|
GUMARC |
49.76 |
52.02 |
2.26 |
| 7 |
Walker, Elise M (7)
|
GUSSAN |
57.77 |
53.13 |
-4.64 |
| 8 |
Taylor, Isabelle R (7)
|
GUSSAN |
53.26 |
53.74 |
0.48 |
| 9 |
Cosmi, Eliyah J (7)
|
GUMARC |
52.90 |
53.91 |
1.01 |
| 10 |
Hadley, Kaitlyn N (7)
|
GUSSAN |
50.56 |
54.73 |
4.17 |
| 11 |
Hallmark, Kaylee G (7)
|
GUSSAN |
1:00.60 |
56.73 |
-3.87 |
| 12 |
Treadaway, Caeleigh R (7)
|
GUMAC |
52.17 |
57.04 |
4.87 |
| 13 |
Coker, Vivienne L (7)
|
GUSSAN |
|
58.58 |
- |
| 14 |
Martinez, Fernanda (7)
|
GUMAC |
|
1:01.11 |
- |
| 15 |
Metzger, Hadley A (7)
|
GUSSAN |
|
1:17.25 |
- |
|
Armand, Ava G (7)
|
GUSSAN |
47.60 |
NS |
- |