| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Otwell, Jaden N (7)
|
GUSSAN |
36.34 |
36.10 |
-0.24 |
| 2 |
Yang, Chloe Y (7)
|
GUSSAN |
47.46 |
49.86 |
2.40 |
| 3 |
Herbel, Camille C (7)
|
GUSSAN |
46.99 |
50.90 |
3.91 |
| 4 |
VanVossen, Kathryn G (7)
|
GUMARC |
52.26 |
51.35 |
-0.91 |
| 5 |
Treadaway, Caeleigh R (7)
|
GUMAC |
1:03.01 |
54.55 |
-8.46 |
| 6 |
Dulworth, Ella M (7)
|
GUSSAN |
|
54.68 |
- |
| 7 |
Walker, Elise M (7)
|
GUSSAN |
1:06.13 |
55.96 |
-10.17 |
| 8 |
Blaylock, Madison L (7)
|
GUMARC |
57.00 |
57.28 |
0.28 |
| 9 |
Hallmark, Kaylee G (7)
|
GUSSAN |
58.99 |
57.97 |
-1.02 |
| 10 |
Griffin-Coyle, Addyson G (7)
|
GUMAC |
1:00.37 |
1:00.38 |
0.01 |
| 11 |
Hadley, Kaitlyn N (7)
|
GUSSAN |
1:01.86 |
1:00.44 |
-1.42 |
| 12 |
Cosmi, Eliyah J (7)
|
GUMARC |
1:01.94 |
1:06.15 |
4.21 |
| 13 |
Heidenreich, Emmalee A (7)
|
GUMAC |
|
1:12.85 |
- |
| 14 |
Martinez, Fernanda (7)
|
GUMAC |
|
1:15.64 |
- |
|
Armand, Ava G (7)
|
GUSSAN |
|
NS |
- |