| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Calone, Emilia R (9)
|
MRMWDA |
36.26 |
37.49 |
1.23 |
| 2 |
Cefaloni, Gabriella J (10)
|
MRAGMV |
39.22 |
38.40 |
-0.82 |
| 3 |
Buliung, Madison V (10)
|
MRAGMV |
44.98 |
43.04 |
-1.94 |
| 4 |
Drackett, Samaya E (10)
|
MRNYSA |
42.05 |
44.04 |
1.99 |
| 5 |
Eskicioglu, Ava J (9)
|
MRAGMV |
49.14 |
44.58 |
-4.56 |
| 6 |
Peters, Gianna M (10)
|
MRNYSA |
46.68 |
47.99 |
1.31 |
| 7 |
McAuley, Nadia A (9)
|
MRAGMV |
51.32 |
51.14 |
-0.18 |
| 8 |
Meyer, Alexis M (9)
|
MRNYSA |
59.79 |
53.31 |
-6.48 |
| 9 |
Sullivan, Emily C (9)
|
MRTRS |
1:01.58 |
54.00 |
-7.58 |
| 10 |
Cabrera, Natalee J (10)
|
MRMWDA |
55.53 |
56.94 |
1.41 |
| 11 |
O'Brien, Abigail C (9)
|
MRAGMV |
56.34 |
57.87 |
1.53 |
| 12 |
McSweeney, Madelyn H (10)
|
MRAGMV |
|
59.16 |
- |