| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Healy, Sierra (12)
|
MRHYB |
36.00 |
34.68 |
-1.32 |
| 2 |
WU, Julian (12)
|
MRLGAC |
34.98 |
34.97 |
-0.01 |
| 3 |
Mendoza, Patricia Victoria G (11)
|
MRFLY |
36.65 |
36.47 |
-0.18 |
| 4 |
Haimson, Emma A (12)
|
MRLGAC |
39.04 |
36.48 |
-2.56 |
| 5 |
Horiuchi, Naomi Z (12)
|
MRLGAC |
36.85 |
36.54 |
-0.31 |
| 6 |
Karim, Azana S (11)
|
MRLGAC |
39.46 |
36.59 |
-2.87 |
| 7 |
LI, Sabrina M (12)
|
MRUN |
|
38.30 |
- |
| 7 |
Jeon, Lauren H (12)
|
MRFLY |
38.05 |
38.30 |
0.25 |
| 9 |
Tesovic, Tamara (11)
|
MRLGAC |
39.66 |
38.37 |
-1.29 |
| 10 |
Henson, Kohana (11)
|
MRMCBY |
40.95 |
39.49 |
-1.46 |
| 11 |
Edlin-Miller, Lia (12)
|
MRHYB |
39.88 |
39.94 |
0.06 |
| 11 |
Tenzin, Quinci R (11)
|
MRLGAC |
42.54 |
39.94 |
-2.60 |
| 13 |
O'Sullivan, Saoirse (12)
|
MRLGAC |
40.07 |
40.25 |
0.18 |
| 14 |
Zheng, Kaitlyn A (12)
|
MRLGAC |
47.28 |
40.55 |
-6.73 |
| 15 |
Mullin, Allison B (12)
|
MRLGAC |
39.97 |
40.84 |
0.87 |
| 16 |
Chan, Megan (11)
|
MRFLY |
41.98 |
41.81 |
-0.17 |
| 17 |
Loftus, Ava E (11)
|
MRCIY |
44.76 |
42.16 |
-2.60 |
| 18 |
Chang, Isabella M (11)
|
MRCIY |
45.85 |
42.47 |
-3.38 |
| 19 |
Rodriguez, Natalia R (11)
|
MRFLY |
44.68 |
42.70 |
-1.98 |
| 20 |
Dragan, Juliana M (12)
|
MRCIY |
43.63 |
42.91 |
-0.72 |
| 21 |
Kohm, Riley S (12)
|
MRCIY |
47.18 |
43.22 |
-3.96 |
| 22 |
Hasimaj, Briana (11)
|
MRMCBY |
44.09 |
43.71 |
-0.38 |
| 23 |
Gyaltsen, Riksung T (11)
|
MRLGAC |
44.25 |
43.76 |
-0.49 |
| 24 |
Thwesakpwkde, Pearl P (12)
|
MRLGAC |
56.43 |
44.13 |
-12.30 |
| 25 |
Miller, Amanda D (12)
|
MRCIY |
50.15 |
47.40 |
-2.75 |
| 26 |
Koch, Samantha W (12)
|
MRLGAC |
50.90 |
47.59 |
-3.31 |
| 27 |
So, Kaileen W (11)
|
MRMCBY |
|
47.78 |
- |
| 28 |
Lama, Renchhen L (11)
|
MRLGAC |
|
47.85 |
- |
| 29 |
Hwang, Gabrielle (12)
|
MRFLY |
|
50.69 |
- |
| 30 |
Brennan, Valentina F (11)
|
MRCIY |
56.82 |
52.94 |
-3.88 |