| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Giannini, Alanah M (10)
|
MRPBAC |
37.46 |
36.00 |
-1.46 |
| 2 |
Cefaloni, Gabriella J (10)
|
MRAGMV |
45.78 |
37.53 |
-8.25 |
| 3 |
Hogger, Annika R (10)
|
MRFA |
39.16 |
39.62 |
0.46 |
| 4 |
Burger, Laura K (10)
|
MRNDAC |
39.55 |
42.55 |
3.00 |
| 5 |
Buliung, Madison V (10)
|
MRAGMV |
41.34 |
43.68 |
2.34 |
| 6 |
Kirshbaum, Sofie M (10)
|
MRSS |
42.15 |
44.81 |
2.66 |
| 7 |
Nakamori, Leah Y (9)
|
MRFA |
51.06 |
48.81 |
-2.25 |
| 8 |
McAuley, Nadia A (9)
|
MRAGMV |
55.42 |
49.74 |
-5.68 |
| 9 |
Walsh, Leah E (9)
|
MRNBS |
51.36 |
51.68 |
0.32 |
| 10 |
Haydt, Abigail G (9)
|
MRNBS |
51.27 |
52.27 |
1.00 |
| 11 |
Jude, Catherine I (10)
|
MRNDAC |
56.80 |
52.94 |
-3.86 |
| 12 |
Sigler, Eva A (9)
|
MRFA |
58.20 |
54.09 |
-4.11 |
| 13 |
Piscitella, Vivian I (10)
|
MRNBS |
56.75 |
56.33 |
-0.42 |
| 14 |
Boehm, Paige M (9)
|
MRNDAC |
1:08.99 |
57.83 |
-11.16 |
| 15 |
Dwek, Sally E (9)
|
MRSSL |
1:07.61 |
1:03.67 |
-3.94 |
| 16 |
Rich, Lauren M (10)
|
MRWSSC |
1:13.15 |
1:10.62 |
-2.53 |
|
Jones-Torres, Olivia-Ziva J (9)
|
MRPBAC |
1:01.23 |
DQ |
- |
|
Ruback, Alena L (10)
|
MRNBS |
|
DQ |
- |
|
Luke, Naomi (9)
|
MRFA |
|
DQ |
- |