| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hill, Sasha (12)
|
MRYMID |
33.38 |
33.09 |
-0.29 |
| 2 |
Park, Kayce H (11)
|
MRNYSA |
33.48 |
33.43 |
-0.05 |
| 3 |
Bruff, Alexandra I (12)
|
MRYMID |
34.19 |
33.48 |
-0.71 |
| 4 |
Trinidad, Mianny L (12)
|
MRRED |
34.22 |
34.07 |
-0.15 |
| 5 |
Leal, Ashley (11)
|
MRRED |
36.06 |
34.33 |
-1.73 |
| 6 |
Auquilla, Litsi D (12)
|
MRRED |
33.24 |
34.75 |
1.51 |
| 7 |
Kyqykaliu, Vesa A (11)
|
MRNYSA |
36.67 |
35.96 |
-0.71 |
| 8 |
Mejia, Jazmine (12)
|
MRYMID |
37.19 |
36.06 |
-1.13 |
| 9 |
DeGeorge, Sadie (11)
|
MRUN |
|
36.11 |
- |
| 10 |
Sarpong, Afia (12)
|
MRHVD |
35.10 |
36.16 |
1.06 |
| 11 |
Johnson, Cayla A (11)
|
MRAPEX |
40.94 |
36.35 |
-4.59 |
| 12 |
Concepcion, Alex P (11)
|
MRNYSA |
35.40 |
36.36 |
0.96 |
| 13 |
Rosario, Michelle D (12)
|
MRRED |
38.14 |
36.40 |
-1.74 |
| 14 |
Dabo, Mariama F (11)
|
MRHVD |
34.12 |
36.99 |
2.87 |
| 15 |
Trubenbach, Grace M (12)
|
MRNYSA |
39.67 |
37.87 |
-1.80 |
| 16 |
Salazar, Emily A (11)
|
MRAPEX |
45.25 |
38.29 |
-6.96 |
| 17 |
Losada, Aleksandra (11)
|
MRNYSA |
39.43 |
38.70 |
-0.73 |
| 18 |
Hegedus, Leda (12)
|
MRRED |
37.99 |
38.77 |
0.78 |
| 19 |
Ha, Kara (12)
|
MRLIFE |
36.59 |
39.00 |
2.41 |
| 20 |
Reyes Aguirre, Juana (11)
|
MRAPEX |
40.88 |
43.58 |
2.70 |
| 21 |
Vaughan, Emma E (12)
|
MRNYSA |
52.84 |
45.76 |
-7.08 |
| 22 |
Brydon, Miranda X (11)
|
MRRED |
47.80 |
48.02 |
0.22 |
|
Arevalo, Jayra N (11)
|
MRRED |
45.31 |
NS |
- |
|
Hendrickson, Riley D (11)
|
MRYMID |
36.49 |
NS |
- |