| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shah, Sun-Jin (10)
|
MRNYCC |
42.29 |
41.91 |
-0.38 |
| 2 |
Mialkovskyi, Denys (10)
|
MRRIST |
47.79 |
46.44 |
-1.35 |
| 3 |
Garcia-Reyes, Anthony (10)
|
CTZEUS |
48.18 |
48.81 |
0.63 |
| 4 |
Karadeniz, Derin M (10)
|
MRPATS |
52.60 |
48.96 |
-3.64 |
| 5 |
Seliger, Timur (9)
|
MRRIST |
46.75 |
49.93 |
3.18 |
| 6 |
Zhu, Aidan (10)
|
MRYFD |
52.26 |
50.78 |
-1.48 |
| 7 |
Van Wormer, Benjamin C (10)
|
MRPATS |
54.76 |
51.55 |
-3.21 |
| 8 |
DeWalle, Adam Z (10)
|
MRYFD |
52.77 |
52.00 |
-0.77 |
| 9 |
Moody, Paul C (10)
|
MRPATS |
57.20 |
52.22 |
-4.98 |
| 10 |
Gonzalez, Alejandro (10)
|
MRAPEX |
51.62 |
52.71 |
1.09 |
| 11 |
Radley, Cole N (10)
|
MRYFD |
55.31 |
54.08 |
-1.23 |
| 12 |
Remor, Ian (10)
|
MRRIST |
52.37 |
55.51 |
3.14 |
| 13 |
Haridasan, Nikolai (9)
|
MRYFD |
1:02.86 |
56.68 |
-6.18 |
| 14 |
Yang, Ryan Y (10)
|
MRYFD |
57.79 |
56.86 |
-0.93 |
| 15 |
Elliott, Chase J (9)
|
CTUN |
1:01.30 |
57.68 |
-3.62 |
| 16 |
Ruiz, Nathaniel R (10)
|
MRHVD |
57.27 |
58.54 |
1.27 |
| 17 |
Chenski, Alexander C (9)
|
MRPATS |
1:01.80 |
59.14 |
-2.66 |
| 18 |
VALENTON, PETER M (9)
|
CTZEUS |
1:00.33 |
59.28 |
-1.05 |
| 19 |
Calero, Maximus A (9)
|
MRYFD |
1:02.82 |
1:01.29 |
-1.53 |
| 20 |
Johnson, Quinn W (10)
|
MRYFD |
1:04.97 |
1:05.60 |
0.63 |
| 21 |
Chavez, Oscar (9)
|
CTZEUS |
1:04.20 |
1:09.67 |
5.47 |
| 22 |
Mora, Noah D (9)
|
CTZEUS |
1:06.22 |
1:09.70 |
3.48 |
| 23 |
Alvares, Matthias M (9)
|
MRAPEX |
1:08.94 |
1:10.17 |
1.23 |
| 24 |
Re, Nicola (10)
|
MRRIST |
1:21.30 |
1:12.86 |
-8.44 |
| 25 |
Chavez, Carlos A (9)
|
MRAPEX |
1:11.47 |
1:15.72 |
4.25 |
| 26 |
Grove, Aiden E (9)
|
MRPATS |
NT |
1:15.79 |
75.79 |
| 27 |
Constancio, Markus A (10)
|
MRNBS |
1:20.59 |
DQ |
- |
| 28 |
Hansel, Eamon J (9)
|
MRPATS |
NT |
DQ |
- |
| 29 |
VanVelzor, Evan P (9)
|
CTZEUS |
50.63 |
NS |
- |