| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dugo, Jesse D (13)
|
MRWISC |
26.98 |
25.78 |
-1.20 |
| 2 |
Dickman, Kellen (14)
|
MRVAC |
26.17 |
25.98 |
-0.19 |
| 3 |
Cepeda, Lorenzo C (13)
|
MRRFAC |
26.37 |
26.37 |
0.00 |
| 4 |
Herbert, Owain G (14)
|
MRNDAC |
25.80 |
26.42 |
0.62 |
| 5 |
Plaza, Sebastian T (14)
|
MRLIE |
26.98 |
26.74 |
-0.24 |
| 6 |
LaBianca, Jack B (13)
|
MRLIE |
26.79 |
26.99 |
0.20 |
| 7 |
Weinberg, Harrison (14)
|
MRLIE |
29.50 |
27.06 |
-2.44 |
| 8 |
Sansano, Matthew A (13)
|
MRLIE |
27.98 |
27.09 |
-0.89 |
| 9 |
Mollica, Christian J (13)
|
MRLIE |
27.27 |
27.19 |
-0.08 |
| 10 |
Murray, Tristan D (14)
|
MRHVD |
29.13 |
27.25 |
-1.88 |
| 11 |
Sherman, Benjamin A (14)
|
MRLIE |
27.02 |
27.30 |
0.28 |
| 12 |
Abramson, Jesse A (13)
|
MRAGMV |
29.13 |
27.34 |
-1.79 |
| 13 |
Clyde, Miles M (13)
|
MRHVD |
27.40 |
27.49 |
0.09 |
| 14 |
Abbruzzese, Matthew J (13)
|
MRRFAC |
27.76 |
27.71 |
-0.05 |
| 15 |
Kukreti, Satvik (14)
|
MRRFAC |
28.01 |
27.92 |
-0.09 |
| 16 |
Burger, Nathan L (13)
|
MRNDAC |
29.05 |
28.14 |
-0.91 |
| 16 |
Schweppe, Otto W (14)
|
MRNDAC |
27.83 |
28.14 |
0.31 |
| 18 |
Harrilal, Ayden B (13)
|
MRLIE |
29.33 |
28.33 |
-1.00 |
| 19 |
Zegadlo, Zachari C (13)
|
MRLIE |
28.45 |
28.56 |
0.11 |
| 20 |
Sullivan, Ryan N (13)
|
MRLIE |
29.00 |
29.03 |
0.03 |
| 21 |
Elmassalemah, Iad K (14)
|
MRNDAC |
29.39 |
30.38 |
0.99 |
| 22 |
Cook, Hunter R (13)
|
MRLIE |
32.30 |
30.48 |
-1.82 |
| 23 |
Barberi, Danny (13)
|
MRLIE |
31.05 |
30.93 |
-0.12 |
| 24 |
Christou, Alex (13)
|
MRLIE |
31.67 |
31.20 |
-0.47 |
| 25 |
Christou, Theo (13)
|
MRLIE |
31.70 |
31.78 |
0.08 |
| 26 |
Blazek, Jackson M (13)
|
MRNDAC |
32.32 |
32.56 |
0.24 |
| 27 |
Stroman Taylor, Jahmair (13)
|
MRMHH |
42.51 |
34.26 |
-8.25 |
| 28 |
Johnson, William F (13)
|
MRHVD |
35.24 |
34.56 |
-0.68 |
| 29 |
Haedrich, James F (13)
|
MRLIE |
35.97 |
35.09 |
-0.88 |
| 30 |
Decicco, Jonathan P (13)
|
MRLIE |
35.24 |
35.55 |
0.31 |