| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ivan, Teresa (14)
|
NEGS |
31.86 |
33.17 |
1.31 |
| 2 |
Acosta McKenzie, Vianka Y (16)
|
NEMTSC |
34.62 |
33.79 |
-0.83 |
| 3 |
Carline, Jill K (14)
|
NEHAY |
33.29 |
34.03 |
0.74 |
| 4 |
Caron, Gabrielle A (17)
|
NEMTSC |
34.00 |
34.13 |
0.13 |
| 5 |
Liang, Mina Y (17)
|
NEMTSC |
34.82 |
35.56 |
0.74 |
| 6 |
Pearson, Sophia E (17)
|
NEMTSC |
33.28 |
35.89 |
2.61 |
| 7 |
Huot, Makenna N (13)
|
NEGS |
38.39 |
35.91 |
-2.48 |
| 8 |
Tcherepanova, Alexandra (14)
|
NEHAY |
40.12 |
38.05 |
-2.07 |
| 9 |
Manning, Madeline E (14)
|
NEGS |
35.84 |
38.11 |
2.27 |
| 10 |
Wands, Erika L (14)
|
NEMTSC |
40.48 |
41.56 |
1.08 |
| 11 |
Morrison, Faith A (16)
|
NEHAY |
43.65 |
41.99 |
-1.66 |
| 12 |
Sinotte, Faye M (13)
|
NEHAY |
47.59 |
42.23 |
-5.36 |
| 13 |
Halpern, Avery E (13)
|
NEMTSC |
43.58 |
43.69 |
0.11 |
| 14 |
Vannelli, Michelle M (14)
|
NEHAY |
48.07 |
44.34 |
-3.73 |
|
Williams, Sophia E (14)
|
NEHAY |
1:03.83 |
DQ |
- |
|
Skala, Lauren M (17)
|
NEMTSC |
32.55 |
NS |
- |