| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Villamil, Ana M (13)
|
FGMWA |
31.56 |
32.16 |
0.60 |
| 2 |
Bellardi, Nicol A (13)
|
FGMWA |
35.97 |
34.78 |
-1.19 |
| 3 |
Osorio, Sabrina (13)
|
FGSOFL |
33.40 |
35.30 |
1.90 |
| 4 |
Fronimakis-Liu, Juliana (13)
|
FGYSF |
36.51 |
35.98 |
-0.53 |
| 5 |
Virto, Mia (13)
|
FGMWA |
35.01 |
36.37 |
1.36 |
| 6 |
Escudero, Mia F (14)
|
FGUNYS |
35.45 |
37.49 |
2.04 |
| 7 |
Ginestra, Isabella (13)
|
FGYSF |
40.37 |
40.85 |
0.48 |
| 8 |
Wooden, Azana (13)
|
FGFLA |
|
41.05 |
- |
| 9 |
Emmanuel, X'Spinziv (14)
|
FGFLA |
|
42.29 |
- |
| 10 |
Bloom, Chloe A (14)
|
FGPAQ |
44.67 |
42.60 |
-2.07 |
| 11 |
Ortiz, Daniela S (13)
|
FGMWA |
|
43.33 |
- |
| 12 |
Zamora, Luciana A (13)
|
FGMWA |
|
43.78 |
- |
| 13 |
Canning, Caroline R (14)
|
FGPAQ |
43.72 |
46.34 |
2.62 |
| 14 |
Kantamneni, Nicole V (13)
|
FGSOFL |
|
49.61 |
- |
|
Osorio, Sofia (13)
|
FGSOFL |
38.61 |
NS |
- |