| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rivera, Samuel T (15)
|
NIIRON |
25.90 |
24.78 |
-1.12 |
| 2 |
Bosa, Andrea G (13)
|
NIPACK |
32.34 |
30.32 |
-2.02 |
| 3 |
Vasta, Myles T (14)
|
NIFAST |
33.01 |
34.68 |
1.67 |
| 4 |
Tang, Howard Z (11)
|
NIPACK |
43.51 |
35.89 |
-7.62 |
| 5 |
Hinds, Benjamin W (10)
|
NIWEBS |
37.27 |
37.17 |
-0.10 |
| 6 |
Soto, Giovanni A (11)
|
NIIRON |
46.13 |
39.27 |
-6.86 |
| 7 |
Kalkounis, GeorgeAnthony (16)
|
NIWEBS |
45.00 |
39.80 |
-5.20 |
| 8 |
Coccia, Bryson A (9)
|
NIGCOM |
44.27 |
40.27 |
-4.00 |
| 9 |
Brett, Liam J (12)
|
NIPACK |
37.57 |
40.93 |
3.36 |
| 10 |
Zappia, Luke H (11)
|
NIPACK |
45.44 |
42.45 |
-2.99 |
| 11 |
Frenzel, Jack W (10)
|
NIWEBS |
46.81 |
43.94 |
-2.87 |
| 12 |
Peters, Ryan R (14)
|
NIGCOM |
50.14 |
46.76 |
-3.38 |
| 13 |
Cunniffe, Terence E (8)
|
NIPACK |
|
47.18 |
- |
| 14 |
Blanchard, Zachary E (12)
|
NIFAST |
46.92 |
50.73 |
3.81 |
| 15 |
Rossetti, Maxwell R (12)
|
NIPACK |
|
57.07 |
- |
| 16 |
Quigley, Braidan T (12)
|
NIWEBS |
1:05.01 |
1:00.91 |
-4.10 |
|
Loomis, Noah W (12)
|
NIIRON |
1:00.00 |
DQ |
- |
|
Cottone, Cameron A (11)
|
NIGCOM |
52.01 |
DQ |
- |
|
Ferrari, Tyler J (13)
|
NIFAST |
|
DQ |
- |