| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mack, Andi M (20)
|
NCUN |
55.50 |
56.34 |
0.84 |
| 2 |
Schimmoller, Jennifer A (15)
|
NCSAIL |
1:01.69 |
1:01.27 |
-0.42 |
| 3 |
Rouzie, Molly C (16)
|
VACGBD |
1:00.55 |
1:01.64 |
1.09 |
| 4 |
Luther, Mattie E (15)
|
NCSAIL |
59.51 |
1:02.86 |
3.35 |
| 5 |
Oettinger, Emily G (16)
|
NCATOM |
1:02.08 |
1:04.03 |
1.95 |
| 6 |
Johnson, Madeleine M (16)
|
NCSAIL |
1:01.97 |
1:05.43 |
3.46 |
| 7 |
Plaza, Sofia M (12)
|
NCATOM |
1:04.70 |
1:05.67 |
0.97 |
| 8 |
Kuhn, Sarah P (17)
|
NCBAD |
1:17.08 |
1:06.13 |
-10.95 |
| 9 |
Prager, Kate E (17)
|
NCSAIL |
1:05.60 |
1:07.13 |
1.53 |
| 10 |
Repella, Allison B (12)
|
NCATOM |
1:10.53 |
1:08.53 |
-2.00 |
| 11 |
Cohn, Olivia J (15)
|
VACGBD |
1:05.72 |
1:10.05 |
4.33 |
| 12 |
Sellner, Emilie M (12)
|
NCATOM |
1:19.23 |
1:18.64 |
-0.59 |