| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schmitz, Madison G (9)
|
NCGFSC |
2:29.98 |
2:25.34 |
-4.64 |
| 2 |
Williams, Lexi M (10)
|
NCWOW |
2:39.14 |
2:40.59 |
1.45 |
| 3 |
Reaves, Camren G (10)
|
NCWOW |
2:41.15 |
2:41.45 |
0.30 |
| 4 |
Rypkema, Ava R (10)
|
NCGFSC |
2:48.60 |
2:41.85 |
-6.75 |
| 5 |
Jacquemin, Madison G (10)
|
SCCAC |
2:52.26 |
2:43.35 |
-8.91 |
| 6 |
Jansky, Savannah L (10)
|
SCCAC |
2:58.38 |
2:47.68 |
-10.70 |
| 7 |
Pace, Isabelle B (9)
|
NCBAC |
2:52.24 |
2:56.05 |
3.81 |
| 8 |
Caron, Tess A (9)
|
NCBAC |
3:12.75 |
2:59.10 |
-13.65 |
| 9 |
Pace, Emileigh D (10)
|
NCBAC |
3:03.88 |
3:01.24 |
-2.64 |
| 10 |
Robideaux, Chandra R (10)
|
SCCAC |
3:03.16 |
3:03.62 |
0.46 |
| 11 |
Santucci, Skylar (10)
|
NCWOW |
3:08.04 |
3:05.51 |
-2.53 |
| 12 |
Adi, Maya (10)
|
SCCAC |
3:14.47 |
3:13.42 |
-1.05 |
| 13 |
Gordon, Kennedy S (9)
|
NCBAC |
3:44.13 |
3:13.51 |
-30.62 |
| 14 |
Ciancanelli, Isabella R (9)
|
NCGFSC |
3:20.31 |
3:13.85 |
-6.46 |
| 15 |
deBidart, Kara P (9)
|
SCMBR |
3:11.19 |
3:18.11 |
6.92 |
| 16 |
Gordon, Harper E (9)
|
NCBAC |
3:42.80 |
3:18.41 |
-24.39 |
| 17 |
Smithwick, Emerson C (9)
|
NCGFSC |
3:25.11 |
3:18.46 |
-6.65 |
| 18 |
Keeler, Samantha B (10)
|
NCWOW |
|
3:54.99 |
- |