| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ai, Bethany R (12)
|
NCCVAC |
40.32 |
37.08 |
-3.24 |
| 2 |
Alvarado-Gamborino, Miranda (12)
|
NCMSC |
47.00 |
41.64 |
-5.36 |
| 3 |
Stewart, Adelee L (12)
|
NCGG |
44.09 |
42.70 |
-1.39 |
| 4 |
Lee, Jovie M (11)
|
NCCVAC |
45.12 |
43.79 |
-1.33 |
| 5 |
Caudle, Riley S (12)
|
NCCVAC |
46.15 |
43.80 |
-2.35 |
| 6 |
Schrock, Annalee K (11)
|
NCWA |
45.95 |
45.37 |
-0.58 |
| 7 |
Gordon, June M (12)
|
NCCVAC |
59.49 |
46.12 |
-13.37 |
| 8 |
Yelton, Lucy E (12)
|
NCMSC |
48.45 |
46.25 |
-2.20 |
| 9 |
Walters, Abigail R (12)
|
NCCVAC |
46.98 |
46.45 |
-0.53 |
| 10 |
Van Meter, Claire V (11)
|
NCCVAC |
47.85 |
46.70 |
-1.15 |
| 11 |
Carroll, Kiersten I (12)
|
NCCVAC |
|
46.94 |
- |
| 12 |
Stewart, Leah M (11)
|
NCCVAC |
50.51 |
47.36 |
-3.15 |
| 13 |
Jones, Reilly E (11)
|
NCWA |
47.80 |
47.44 |
-0.36 |
| 14 |
Alvarez, Dayana N (11)
|
NCCVAC |
1:08.19 |
48.69 |
-19.50 |
| 15 |
Floyd, Lilly G (11)
|
NCGG |
50.94 |
48.97 |
-1.97 |
| 16 |
Seraz, Larissa F (11)
|
NCCVAC |
|
51.09 |
- |
| 17 |
Finney, Merrill P (11)
|
NCCVAC |
55.11 |
51.57 |
-3.54 |
| 18 |
Greene, Jordyn A (11)
|
NCMSC |
52.36 |
53.14 |
0.78 |
| 19 |
White, Skyler R (11)
|
NCCVAC |
58.66 |
53.34 |
-5.32 |
| 20 |
Negrete-Perez, Stephanie (12)
|
NCCVAC |
52.57 |
53.43 |
0.86 |
| 21 |
Kale, Zelina M (12)
|
NCCVAC |
56.10 |
57.71 |
1.61 |
|
Ramirez-Soto, Vanessa (12)
|
NCCVAC |
|
DQ |
- |
|
Li, Rui T (11)
|
NCAQTC |
|
DQ |
- |
|
Rodriquez, Maria V (12)
|
NCMSC |
44.09 |
NS |
- |