| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lawless, Ethan J (16)
|
NCECA |
50.95 |
50.06 |
-0.89 |
| 2 |
Cook, Turner M (15)
|
NCECA |
51.28 |
51.50 |
0.22 |
| 3 |
Tillery, Seth R (17)
|
NCECA |
51.16 |
52.45 |
1.29 |
| 4 |
Pake, Tristan G (15)
|
NCECA |
53.70 |
52.81 |
-0.89 |
| 5 |
Bryant, Tyler J (15)
|
NCECA |
54.77 |
54.99 |
0.22 |
| 6 |
Cassell, Morgan C (16)
|
NCECA |
1:04.14 |
58.06 |
-6.08 |
| 7 |
Hatfield, Geoffrey T (13)
|
NCECA |
1:00.00 |
59.27 |
-0.73 |
| 8 |
Smith, Jacob A (16)
|
NCECA |
59.32 |
1:00.55 |
1.23 |
| 9 |
Taylor, Kai A (13)
|
NCECA |
1:12.65 |
1:04.26 |
-8.39 |
| 10 |
Clifford, Ethan C (13)
|
NCECA |
1:05.76 |
1:06.53 |
0.77 |
| 11 |
Taylor, Lukas O (13)
|
NCECA |
1:13.25 |
1:06.71 |
-6.54 |
| 12 |
Law, Cooper J (13)
|
NCECA |
|
1:09.59 |
- |
| 13 |
Marlow, Tiago V (12)
|
NCECA |
1:09.97 |
1:11.07 |
1.10 |
| 14 |
Johnson, Samuel T (11)
|
NCECA |
1:16.89 |
1:14.41 |
-2.48 |
| 15 |
Cobb, Adyn M (13)
|
NCECA |
1:22.27 |
1:18.11 |
-4.16 |
| 16 |
Sass, Elliott J (11)
|
NCECA |
|
1:18.84 |
- |
| 17 |
Squires, Arthur James M (12)
|
NCECA |
1:33.18 |
1:22.38 |
-10.80 |
| 18 |
Manuel, Conrad R (8)
|
NCECA |
|
1:26.77 |
- |
| 19 |
Blaylock, Dakota M (11)
|
NCECA |
1:37.62 |
1:39.15 |
1.53 |
|
Smith, Sadler V (16)
|
NCECA |
49.08 |
DQ |
- |
|
Lundberg, Stephen P (17)
|
NCECA |
50.30 |
NS |
- |
|
Terrazas, Johnny (13)
|
NCECA |
1:25.70 |
NS |
- |