| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Keefe, Paige A (12)
|
NCYOTA |
30.94 |
30.94 |
0.00 |
| 2 |
Coates, Nina A (12)
|
NCNEAL |
33.37 |
32.58 |
-0.79 |
| 3 |
Wunderlich, Peyton M (12)
|
NCECA |
34.78 |
35.99 |
1.21 |
| 4 |
Miller, Lorelai M (11)
|
NCECA |
40.93 |
36.74 |
-4.19 |
| 5 |
Christian, Allison C (12)
|
NCECA |
37.50 |
37.49 |
-0.01 |
| 6 |
Blackmon, Bryn E (12)
|
NCECA |
38.76 |
39.31 |
0.55 |
| 7 |
Harper, Gabbrielle E (12)
|
NCGOLD |
40.99 |
40.19 |
-0.80 |
| 8 |
Kilgallen, Kathryn E (12)
|
NCYOTA |
40.80 |
40.86 |
0.06 |
| 9 |
Nelson, Grace E (11)
|
NCNEAL |
50.76 |
42.32 |
-8.44 |
| 10 |
Greaves, London C (12)
|
NCNEAL |
42.60 |
42.73 |
0.13 |
| 11 |
Flanagan, Madeleine G (12)
|
NCNEAL |
|
43.44 |
- |
| 12 |
McIntyre, Taylor J (11)
|
NCECA |
54.03 |
50.00 |
-4.03 |
|
Mixa, Olivia S (12)
|
NCNEAL |
39.27 |
NS |
- |