| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McChesney, Shelby K (14)
|
NCTYDE |
26.00 |
26.98 |
0.98 |
| 2 |
Evans, Victoria G (13)
|
NCGCY |
26.63 |
27.05 |
0.42 |
| 3 |
Morrow, Amelia G (11)
|
NCEAC |
26.65 |
27.10 |
0.45 |
| 4 |
Rider, Natalie L (14)
|
NCEAC |
28.34 |
27.46 |
-0.88 |
| 5 |
Tingen, Casey L (13)
|
VAWAC |
26.26 |
27.53 |
1.27 |
| 6 |
Pawa, Saanvi (14)
|
NCEAC |
27.30 |
27.58 |
0.28 |
| 7 |
Cranford, Aurora G (14)
|
NCGCY |
26.88 |
27.70 |
0.82 |
| 8 |
Greene, Elizabeth A (14)
|
NCGCY |
26.90 |
27.77 |
0.87 |
| 9 |
McMahan, Ainsley G (13)
|
VAWAC |
28.92 |
27.94 |
-0.98 |
| 10 |
Hanks, Lindsay N (13)
|
VAWAC |
29.01 |
28.05 |
-0.96 |
| 11 |
Maxey, Amelia P (14)
|
NCEAC |
27.83 |
28.09 |
0.26 |
| 12 |
Kohrt, Lucy E (14)
|
NCTYDE |
27.24 |
28.15 |
0.91 |
| 13 |
Williams, Jordan G (12)
|
NCGCY |
27.69 |
28.17 |
0.48 |
| 14 |
Anderson, Chloe E (14)
|
VAWAC |
27.49 |
28.42 |
0.93 |
| 15 |
Wiles, Morgan E (13)
|
NCTYDE |
27.68 |
28.48 |
0.80 |
| 16 |
Pawa, Anika R (11)
|
NCTYDE |
29.56 |
29.00 |
-0.56 |
| 17 |
Brown, Keegan H (12)
|
NCTYDE |
28.42 |
29.68 |
1.26 |
| 18 |
Bollinger, Abigail D (13)
|
VAWAC |
31.92 |
29.77 |
-2.15 |
| 19 |
Tiganus, Sara (13)
|
NCGCY |
|
35.21 |
- |
|
Hodge, Emma C (13)
|
VACCA |
36.23 |
NS |
- |
|
Mann, Kenzie B (13)
|
NCEAC |
29.65 |
NS |
- |
|
Lack, Emilia M (13)
|
NCEAC |
36.49 |
NS |
- |
|
Sessor, Clara F (13)
|
VACCA |
33.62 |
NS |
- |
|
Reichardt, Caroline N (11)
|
VACCA |
47.89 |
NS |
- |