| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mohr, Grace K (14)
|
NCTYDE |
2:17.24 |
2:23.81 |
6.57 |
| 2 |
Osborne, Hadley P (13)
|
NCGCY |
2:42.17 |
2:31.61 |
-10.56 |
| 3 |
Anderson, Chloe E (14)
|
VAWAC |
2:31.46 |
2:33.03 |
1.57 |
| 4 |
Frondoza, Audrianne R (13)
|
NCTYDE |
|
2:33.76 |
- |
| 5 |
Hanks, Lindsay N (13)
|
VAWAC |
2:33.83 |
2:34.25 |
0.42 |
| 6 |
Wiles, Morgan E (13)
|
NCTYDE |
2:37.48 |
2:34.82 |
-2.66 |
| 7 |
McGonigal, Maeren R (13)
|
NCUN |
2:39.99 |
2:34.87 |
-5.12 |
| 8 |
Pawa, Saanvi (14)
|
NCEAC |
2:28.00 |
2:35.09 |
7.09 |
| 9 |
Tingen, Casey L (13)
|
VAWAC |
2:33.28 |
2:37.46 |
4.18 |
| 10 |
Brooks, Alexandria I (12)
|
NCGCY |
2:41.90 |
2:39.12 |
-2.78 |
| 11 |
Kohrt, Lucy E (14)
|
NCTYDE |
2:54.61 |
2:50.47 |
-4.14 |
| 12 |
Goodman, Mary C (14)
|
NCEAC |
3:30.00 |
2:58.73 |
-31.27 |
|
Mann, Kenzie B (13)
|
NCEAC |
3:23.84 |
NS |
- |
|
Greene, Elizabeth A (14)
|
NCGCY |
2:31.70 |
NS |
- |