| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Betancourt, Amelia H (11)
|
NCLOY |
46.08 |
41.86 |
-4.22 |
| 2 |
Osborne, Hadley P (12)
|
NCGCY |
42.14 |
42.08 |
-0.06 |
| 4 |
Brown, Keegan H (11)
|
NCTYDE |
44.01 |
43.20 |
-0.81 |
| 5 |
Bellissimo, Sophia G (11)
|
NCLOY |
47.91 |
44.22 |
-3.69 |
| 6 |
Yeh, Sabrina T (12)
|
NCLOY |
49.31 |
44.93 |
-4.38 |
| 7 |
Nelson, Avery L (12)
|
NCTYDE |
45.16 |
45.32 |
0.16 |
| 8 |
McDonough, Addyson R (11)
|
NCLOY |
48.46 |
46.18 |
-2.28 |
| 9 |
Brewer, Kamryn B (12)
|
NCLOY |
47.83 |
47.17 |
-0.66 |
| 10 |
Schell, Gabrielle T (11)
|
NCGCY |
48.15 |
47.88 |
-0.27 |
| 12 |
Kennedy, Laura K (11)
|
NCTYDE |
51.03 |
48.08 |
-2.95 |
| 13 |
Wiechers, Abigail F (11)
|
NCLOY |
|
49.39 |
- |
| 14 |
Seifert, Camille I (11)
|
NCLOY |
59.33 |
50.37 |
-8.96 |
| 15 |
Moehrke, Brooke A (11)
|
NCTYDE |
53.07 |
50.67 |
-2.40 |
| 16 |
Breeden, Zoey I (12)
|
NCLOY |
|
50.70 |
- |
| 17 |
Malloy, Adrienne S (12)
|
NCLOY |
52.35 |
50.79 |
-1.56 |
| 18 |
McDonald, Anna G (11)
|
NCTYDE |
54.72 |
51.65 |
-3.07 |
| 20 |
Carpenter, Addison L (11)
|
NCLOY |
|
52.23 |
- |
| 21 |
Okoniewski, Kayla M (11)
|
NCLOY |
49.99 |
53.73 |
3.74 |
| 22 |
Daniel, Melanie A (11)
|
NCGCY |
58.56 |
53.80 |
-4.76 |
| 23 |
Hill, Kipley L (12)
|
NCGCY |
56.16 |
54.75 |
-1.41 |
| 24 |
Hunt, Laura G (12)
|
NCGCY |
55.41 |
54.88 |
-0.53 |
| 25 |
Lewis, Piper A (12)
|
NCTYDE |
52.16 |
55.11 |
2.95 |
| 27 |
Parui, Noyonika D (12)
|
NCLOY |
|
58.85 |
- |
| 29 |
Hua, Claire S (11)
|
NCGCY |
59.59 |
1:01.54 |
1.95 |
| 30 |
Koontz, Berkley M (11)
|
NCTYDE |
1:07.77 |
1:04.71 |
-3.06 |
|
Filiault, Julia K (11)
|
NCLOY |
55.52 |
NS |
- |