| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Burton, Piper I (16)
|
NCGCY |
35.60 |
36.87 |
1.27 |
| 3 |
Lin, Jennings E (15)
|
NCGCY |
38.82 |
38.04 |
-0.78 |
| 4 |
Bunting, Meredith T (16)
|
NCTYDE |
39.41 |
38.53 |
-0.88 |
| 5 |
Campbell, McKenzie N (16)
|
NCGCY |
|
38.83 |
- |
| 6 |
Joyce, Alaina M (18)
|
NCGCY |
36.38 |
40.77 |
4.39 |
| 7 |
Jones, Paige L (17)
|
NCTYDE |
40.18 |
42.14 |
1.96 |
| 8 |
Thomas, Camden O (15)
|
NCGCY |
46.53 |
43.09 |
-3.44 |
| 10 |
Sorjonen, Taika A (16)
|
NCGCY |
46.12 |
46.17 |
0.05 |
| 11 |
Salthouse, Hayley K (15)
|
NCGCY |
42.56 |
47.11 |
4.55 |
| 12 |
Hoover, Caroline M (16)
|
NCGCY |
44.03 |
47.89 |
3.86 |
| 13 |
Aldridge, Olivia A (16)
|
NCLOY |
47.97 |
48.56 |
0.59 |
|
Gilliland, Virginia A (18)
|
NCGCY |
34.68 |
NS |
- |
|
Wolf, Anna K (15)
|
NCTYDE |
37.16 |
NS |
- |