| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wilson, Addison R (8)
|
NCWST |
48.78 |
42.89 |
-5.89 |
| 2 |
Harmon, Elizabeth G (8)
|
NCATOM |
|
48.33 |
- |
| 3 |
Barrick, Riley D (7)
|
NCSAC |
|
52.92 |
- |
| 4 |
Mai, Jamie V (8)
|
NCATOM |
55.86 |
55.77 |
-0.09 |
| 5 |
Handy, Elsie R (8)
|
NCCVAC |
59.64 |
56.90 |
-2.74 |
| 6 |
Willer, Avery L (8)
|
NCGG |
|
57.30 |
- |
| 7 |
Miller, Brinley J (8)
|
NCNMA |
|
58.13 |
- |
| 8 |
Davis, Laurel E (8)
|
NCAQTC |
1:01.00 |
1:00.18 |
-0.82 |
| 9 |
Hill, Elise S (8)
|
NCNMA |
|
1:00.25 |
- |
| 10 |
Bennett, Addison R (8)
|
NCRCST |
57.80 |
1:00.70 |
2.90 |
| 11 |
Johnson, Valerie D (6)
|
NCCVAC |
|
1:01.87 |
- |
| 12 |
Veera, Seehe (8)
|
NCNMA |
|
1:04.87 |
- |
| 13 |
Bullins, Blakeney E (8)
|
NCATOM |
1:08.41 |
1:07.11 |
-1.30 |
| 14 |
Huffman, Madison L (7)
|
NCCVAC |
|
1:09.10 |
- |
| 15 |
Stancil, Ellie G (7)
|
NCGG |
|
1:17.73 |
- |
| 16 |
Bartlett, Veronica D (7)
|
NCCVAC |
|
1:17.94 |
- |
| 17 |
Cantu, Camila (8)
|
NCRCST |
|
1:19.26 |
- |
|
Millsaps, Katherine G (6)
|
NCCVAC |
|
DQ |
- |