| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wasson, Hannah L (8)
|
NCCVAC |
39.91 |
41.90 |
1.99 |
| 2 |
Bennett, Addison R (8)
|
NCRCST |
46.05 |
46.47 |
0.42 |
| 3 |
Millsaps, Katherine G (6)
|
NCCVAC |
52.79 |
49.72 |
-3.07 |
| 4 |
Farris, Gracie L (8)
|
NCMSC |
45.18 |
52.47 |
7.29 |
| 5 |
Oglesby, Lily K (8)
|
NCRCST |
|
1:01.31 |
- |
| 6 |
Oglesby, Sadie A (7)
|
NCRCST |
|
1:01.94 |
- |
| 7 |
Huffman, Madison L (7)
|
NCCVAC |
1:00.44 |
1:03.72 |
3.28 |
| 8 |
Davidson, Mila (7)
|
NCMSC |
|
1:04.41 |
- |
| 9 |
Faz, Aleeza L (8)
|
NCRCST |
|
1:05.00 |
- |
| 10 |
Guzman, Mia G (7)
|
NCCVAC |
|
1:05.18 |
- |
| 11 |
Killian, Ashton B (8)
|
NCCVAC |
|
1:08.00 |
- |
| 12 |
Bartlett, Veronica D (7)
|
NCCVAC |
1:08.05 |
1:11.69 |
3.64 |
| 13 |
Beltran-Trejo, Fernanda (6)
|
NCCVAC |
1:07.56 |
1:13.40 |
5.84 |
| 14 |
Leonard, Lillian (8)
|
NCMSC |
|
1:25.04 |
- |