| # | Swimmer | Team | Seed | Time | Diff |
| 2 |
Smith, Austin W (8)
|
MDBCSC |
43.78 |
42.84 |
-0.94 |
| 3 |
Cason, Syeed L (7)
|
CATRA |
43.14 |
42.94 |
-0.20 |
| 4 |
White, Andrew J (8)
|
NCRSA |
46.17 |
46.97 |
0.80 |
| 5 |
Veves, Panagiotis D (8)
|
NCRSA |
53.00 |
47.69 |
-5.31 |
| 6 |
McGee, Joshua X (8)
|
VADIG |
50.64 |
48.18 |
-2.46 |
| 7 |
Garcia, Christian G (8)
|
NJBGCD |
51.38 |
48.42 |
-2.96 |
| 8 |
Cooper, Ryland A (7)
|
MDFMSC |
50.33 |
50.20 |
-0.13 |
| 9 |
Culpepper, Xavier T (8)
|
MIDRST |
53.21 |
51.07 |
-2.14 |
| 10 |
Sumpter, Ethan (8)
|
MDYRAC |
1:09.95 |
52.28 |
-17.67 |
| 12 |
Rodgers, Khalil A (7)
|
MIDRST |
1:03.84 |
55.50 |
-8.34 |
| 13 |
McClam, Marion C (7)
|
NCYSST |
1:06.10 |
56.56 |
-9.54 |
| 14 |
Anderson, Mason W (7)
|
MIRAZ |
54.74 |
57.46 |
2.72 |
| 15 |
Langford, Izeal E (7)
|
MDFMSC |
55.16 |
58.54 |
3.38 |
| 16 |
Dorsey, Christopher L (8)
|
PVERSC |
53.65 |
59.34 |
5.69 |
| 18 |
Harris, Anthony P (7)
|
MIDRST |
54.46 |
1:02.23 |
7.77 |
| 19 |
Johnson, Joshua B (7)
|
MDYRAC |
|
1:03.65 |
- |
| 21 |
Lumumba, Sokoro F (8)
|
MDCSAC |
|
1:06.45 |
- |
| 22 |
Colbert, Adrian c (8)
|
GAHLHK |
1:06.93 |
1:06.95 |
0.02 |
| 23 |
Waddy, Ethan A (6)
|
ILCF |
|
1:07.25 |
- |
| 24 |
Tinsley-Bradford, Aaron D (4)
|
MIRAZ |
1:02.14 |
1:07.74 |
5.60 |
| 26 |
Brown, III, Gregory (4)
|
MDFMSC |
|
2:38.92 |
- |
|
Gomes, Micah A (7)
|
NEFLA |
|
DQ |
- |
|
Braxton, Jacob W (6)
|
MDFMSC |
1:13.65 |
DQ |
- |
|
Brown Jr., Benjamin (8)
|
MIDRST |
1:01.81 |
NS |
- |