| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huecker, Madison D (13)
|
NCMSA |
2:59.35 |
2:58.68 |
-0.67 |
| 2 |
Meier, Anna J (14)
|
NCATOM |
3:06.77 |
3:06.81 |
0.04 |
| 3 |
Reibel, Janie L (13)
|
NCATOM |
3:11.97 |
3:10.13 |
-1.84 |
| 4 |
Kudela, Katie A (13)
|
NCMSA |
3:10.40 |
3:10.92 |
0.52 |
| 5 |
Bambauer, Sophia A (13)
|
NCTEAM |
|
3:24.37 |
- |
| 6 |
Challa, Malli R (13)
|
NCHAC |
3:30.99 |
3:26.32 |
-4.67 |
| 7 |
Cloar, Olivia C (14)
|
NCCVAC |
3:38.15 |
3:27.22 |
-10.93 |
| 8 |
Francis, Jet S (13)
|
NCMSA |
|
3:27.65 |
- |
| 9 |
Parrish, Courtney S (14)
|
NCMSA |
|
3:30.03 |
- |
| 10 |
Drummond, Lena A (13)
|
NCTEAM |
|
3:42.80 |
- |
| 11 |
Cuddy, Lauren A (14)
|
NCMSA |
3:54.60 |
3:43.10 |
-11.50 |
| 12 |
Bastola, Kunjal (14)
|
NCMSA |
4:06.37 |
3:49.85 |
-16.52 |
| 13 |
Harman, Samantha M (14)
|
NCTEAM |
|
3:50.21 |
- |
| 14 |
Narcisse, Alexandria (14)
|
NCQCD |
3:52.92 |
3:53.20 |
0.28 |
| 15 |
Atkins, Hannah M (13)
|
SCUN |
|
4:01.87 |
- |
|
Duckworth, Heather M (14)
|
NCMSA |
3:03.47 |
NS |
- |