| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tars, Robert M (14)
|
NCGCY |
26.19 |
25.97 |
-0.22 |
| 2 |
Holian, Parker E (14)
|
NCSGSA |
28.37 |
27.53 |
-0.84 |
| 3 |
Charles, Miles L (14)
|
NCNCAC |
27.55 |
27.66 |
0.11 |
| 4 |
Chang, Austin W (14)
|
NCNCAC |
28.25 |
28.56 |
0.31 |
| 5 |
Rock, Noah L (13)
|
NCGCY |
29.13 |
29.05 |
-0.08 |
| 6 |
Honore, Alfred N (13)
|
NCNCAC |
27.99 |
29.14 |
1.15 |
| 7 |
Pierce, Brice J (13)
|
MREAST |
30.93 |
30.18 |
-0.75 |
| 8 |
Miroshnichenko, Alexander A (14)
|
NCGCY |
33.57 |
30.35 |
-3.22 |
| 9 |
Reano, Juan F (14)
|
PVSNOW |
30.51 |
31.02 |
0.51 |
| 10 |
Blenk, Gavin M (14)
|
MREAST |
30.90 |
31.12 |
0.22 |
| 11 |
Rodriguez, Alexander R (13)
|
NCGCY |
35.37 |
31.15 |
-4.22 |
| 12 |
Teames, Ethan N (13)
|
PVSNOW |
44.46 |
33.39 |
-11.07 |
| 13 |
Laliberte, Casey J (13)
|
NESSC |
33.95 |
33.62 |
-0.33 |
| 14 |
Roling Stella, Vicenzo (14)
|
NCGCY |
35.99 |
33.79 |
-2.20 |
| 15 |
Rivera, Gabriel J (13)
|
MREAST |
37.79 |
34.58 |
-3.21 |
| 16 |
Tyson, Samuel E (13)
|
PVSNOW |
|
38.03 |
- |
|
Zhang, Ray (14)
|
NCGCY |
41.50 |
NS |
- |
|
Gustafson, Philip C (13)
|
NCACCH |
37.27 |
NS |
- |
|
Evenko, Nicholas (14)
|
MRTRI |
31.42 |
NS |
- |