| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Teague, Estelle M (11)
|
NCSTAR |
39.28 |
37.98 |
-1.30 |
| 2 |
Grubb, Cassidy H (13)
|
NCSTAR |
|
38.12 |
- |
| 3 |
Bryant, Kara E (13)
|
NCSTAR |
41.12 |
39.00 |
-2.12 |
| 4 |
Cooper, Orli L (12)
|
NCSTAR |
|
40.92 |
- |
| 5 |
Neighbors, Kaylie E (13)
|
NCSFSC |
43.68 |
43.39 |
-0.29 |
| 6 |
Grubb, Ashlyn R (11)
|
NCSTAR |
47.83 |
44.24 |
-3.59 |
| 7 |
Barnett, Anna H (12)
|
NCSFSC |
46.46 |
45.29 |
-1.17 |
| 8 |
Jorgenson, Brooke A (10)
|
NCSFSC |
|
45.91 |
- |
| 9 |
Jones, Eloise E (9)
|
NCSFSC |
53.40 |
46.07 |
-7.33 |
| 10 |
Strasser, Morgan G (10)
|
NCSFSC |
46.01 |
46.15 |
0.14 |
| 11 |
Matthews, Sydney L (12)
|
NCSFSC |
|
47.41 |
- |
| 12 |
Stevens, Katherine S (8)
|
NCSTAR |
46.57 |
48.65 |
2.08 |
| 13 |
Risinger, Clara W (10)
|
NCSFSC |
|
49.46 |
- |
| 14 |
Meder, Reagan M (11)
|
NCSFSC |
50.51 |
49.50 |
-1.01 |
| 15 |
Singleton, Julianna M (9)
|
NCSTAR |
57.65 |
50.80 |
-6.85 |
| 16 |
Holmes, Aliza M (9)
|
NCSFSC |
53.85 |
51.45 |
-2.40 |
| 17 |
Sakalouskaya, Valerie G (8)
|
NCSTAR |
51.74 |
52.46 |
0.72 |
| 18 |
Hawks, Kali J (10)
|
NCSTAR |
|
52.91 |
- |
| 19 |
Ault, Serenity E (9)
|
NCSFSC |
53.48 |
52.98 |
-0.50 |
| 20 |
Kuennen, Madeline G (10)
|
NCSTAR |
|
54.80 |
- |
| 21 |
Matthews, Cameron E (9)
|
NCSFSC |
1:00.35 |
54.87 |
-5.48 |
| 21 |
Wieting, Reagan J (9)
|
NCSTAR |
57.91 |
54.87 |
-3.04 |
| 23 |
O'Day, Jenna (9)
|
NCSTAR |
|
55.12 |
- |
| 24 |
Seijn, Kate H (10)
|
NCSFSC |
|
55.67 |
- |
| 25 |
Woodall, Riley M (13)
|
NCSTAR |
|
57.51 |
- |
| 26 |
Krumroy, Ainsley E (10)
|
NCSFSC |
1:01.10 |
1:00.11 |
-0.99 |
| 27 |
Richardson, Sabina R (10)
|
NCSFSC |
|
1:02.45 |
- |
| 28 |
Fulton, Charley G (10)
|
NCSFSC |
1:06.47 |
1:02.73 |
-3.74 |
| 29 |
Teague, Rowan C (6)
|
NCSTAR |
1:15.57 |
1:06.97 |
-8.60 |
| 30 |
Conklin, Highland S (9)
|
NCSFSC |
1:26.06 |
1:24.11 |
-1.95 |
|
Gill, Anna K (9)
|
NCSFSC |
|
NS |
- |