| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Miller, Luke R (12)
|
NCMSA |
35.83 |
35.50 |
-0.33 |
| 2 |
Teoh, Reid P (12)
|
NCSTAR |
36.32 |
36.40 |
0.08 |
| 3 |
James, Alexander M (12)
|
NCMSA |
36.47 |
36.74 |
0.27 |
| 4 |
Garmon, Alexander S (12)
|
NCMAC |
37.22 |
36.90 |
-0.32 |
| 5 |
Gibbs, Mason J (12)
|
NCSAIL |
38.16 |
36.92 |
-1.24 |
| 6 |
Callard, Nathaniel R (12)
|
NCMAC |
39.06 |
38.79 |
-0.27 |
| 7 |
Krisko, James S (12)
|
NCTEAM |
39.69 |
39.24 |
-0.45 |
| 8 |
Thompson, Lucas W (12)
|
NCTEAM |
40.34 |
39.35 |
-0.99 |
| 9 |
Friese, Benjamin L (12)
|
NCMAC |
46.31 |
42.53 |
-3.78 |
| 10 |
McKinnon, Alexander S (12)
|
NCMSA |
42.80 |
42.69 |
-0.11 |
| 11 |
Bright, Owen H (12)
|
NCSAIL |
44.25 |
46.56 |
2.31 |
| 12 |
Waide, Wayman M (12)
|
NCMSA |
|
48.03 |
- |
| 13 |
Hermans, Samuel A (12)
|
NCSAIL |
51.11 |
49.41 |
-1.70 |
|
Monahan, Harrison V (12)
|
NCMAC |
36.27 |
NS |
- |