| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fletcher, Joshua A (17)
|
NCMAC |
46.65 |
46.17 |
-0.48 |
| 2 |
Piscitelli, Nicholas J (15)
|
NCMAC |
47.11 |
47.79 |
0.68 |
| 3 |
Rothenberger, Charles A (17)
|
NCMAC |
46.71 |
47.89 |
1.18 |
| 4 |
Murray, Jeffrey A (19)
|
NCMAC |
45.49 |
48.02 |
2.53 |
| 5 |
Das, Avijit R (15)
|
NCMAC |
48.71 |
48.85 |
0.14 |
| 6 |
Root, Hunter E (18)
|
NCMAC |
49.38 |
49.81 |
0.43 |
| 7 |
Dabhade, Arpeet T (18)
|
NCMAC |
49.93 |
50.12 |
0.19 |
| 8 |
Ford, Nathan F (16)
|
NCMAC |
51.68 |
51.14 |
-0.54 |
| 9 |
Chestnutt, Ryan L (17)
|
NCMAC |
49.93 |
51.34 |
1.41 |
| 10 |
Frye, Thomas J (15)
|
NCMAC |
50.65 |
51.45 |
0.80 |
| 11 |
Kroll, John T (11)
|
NCMAC |
58.64 |
57.97 |
-0.67 |
| 12 |
Munro-Tavormina, Austin M (16)
|
NCUNAT |
|
59.51 |
- |