| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trexler, Grant M (10)
|
NCTAC |
41.76 |
43.62 |
1.86 |
| 2 |
Lu, Matthrew (10)
|
NCTAC |
46.90 |
45.57 |
-1.33 |
| 3 |
Suphavadeprasit, Arman (10)
|
NCTAC |
48.69 |
46.69 |
-2.00 |
| 4 |
Rothchild, Brennan T (10)
|
NCTAC |
47.60 |
47.17 |
-0.43 |
| 5 |
Hignite, Ian G (9)
|
NCTAC |
53.98 |
48.33 |
-5.65 |
| 6 |
Da Silva, Nicholas S (10)
|
NCASTF |
49.93 |
48.43 |
-1.50 |
| 7 |
Yun, Matthew R (10)
|
NCTAC |
47.84 |
48.74 |
0.90 |
| 8 |
Benzing, William D (10)
|
NCTAC |
45.42 |
49.13 |
3.71 |
| 9 |
Pendleton, Zackary A (10)
|
NCGFSC |
49.03 |
50.77 |
1.74 |
| 10 |
Wang, Zian W (9)
|
NCTAC |
56.34 |
54.35 |
-1.99 |
| 11 |
March, Brady A (7)
|
NCTAC |
59.08 |
57.10 |
-1.98 |
| 12 |
Ruhlin, Benjamin G (10)
|
NCTAC |
57.23 |
58.97 |
1.74 |
| 13 |
Collins, Blake D (9)
|
NCGFSC |
1:00.50 |
1:00.42 |
-0.08 |
| 14 |
Brown, Jayden M (8)
|
NCTAC |
|
1:00.74 |
- |
| 15 |
Smith, Jeremiah C (7)
|
NCGFSC |
1:12.19 |
1:15.52 |
3.33 |
|
Palamar, Jonathan P (8)
|
NCTAC |
49.62 |
DQ |
- |
|
Ju, Joshua E (10)
|
NCTAC |
48.75 |
DQ |
- |
|
Vershinskii, Dmitrii (7)
|
NCTAC |
1:00.85 |
NS |
- |
|
Zheng, Adam (10)
|
NCTAC |
38.65 |
NS |
- |